微波辅助提取贯叶连翘总黄酮的工艺研究  

Study on Extracting Total Flavonoids from Hypericum perfortum L. with Microwave-assisted Extraction

作　　者：王小华[1] 邓斌[1,2] 张晓军[2] 龙石红[3] 王存嫦[1] 

机构地区：[1]湘南学院化学与生命科学系,湖南郴州423000 [2]中国科学技术大学合肥微尺度物质科学国家实验室,安徽合肥230026 [3]永州职业技术学院,湖南永州425000

出　　处：《安徽农业科学》2009年第14期6431-6432,6509,共3页Journal of Anhui Agricultural Sciences

基　　金：湖南省自然科学基金资助项目(07JJ3017);湖南省教育厅优秀青年科研基金项目(06B089)

摘　　要：[目的]探讨贯叶连翘中总黄酮的微波辅助提取优化工艺条件。[方法]采用正交试验方法,考察微波功率、提取溶剂浓度、液固比、提取时间对总黄酮含量的影响,确定微波辅助提取贯叶连翘总黄酮的最佳工艺条件,并与超声波提取法、索氏提取法进行比较。[结果]微波辅助提取贯叶连翘总黄酮的最佳条件为乙醇溶液的体积浓度为70%、液固比为30∶1、微波功率450 W、微波提取时间5 min,在该条件下贯叶连翘总黄酮得率为6.02%。微波辅助提取5 m in与索氏法提取4 h、超声波法提取1 h得率相当。[结论]微波辅助提取法具有快速、高效、节能、选择性好等特点。[ Objective ] The study aims to discuss the optimum technological conditions of extracting total flavonoids from Hypericum perfortum L. with microwave-assisted extraction. [ Method] Microwave power, extraction solvent concentration, liquid-solid ratio, and extraction time are investigated and the appropriate microwave-assisted exteaction conditions are obtained with the orthogonal test method. The microwave-assisted exteaction method is compared with ultrasonic extraction and soxhlet extraction methods. [ Result] The optimized conditions of microwave-assisted extraction are as follows： ethanol concentration is 70 %, liquid-solid ratio is 30：1, microwave power is 450 W, and extraction time is 5 minutes. Also the extracting yield of flavoniods from Hypericum perfortum L. is up to 6.02 % under the optimal extraction conditions. The yield of total flavonoids is almost equal to soxhlet extraction method for 4 hours, and ultrasonic extraction method for 1 hours. [ Conclusion ] The microwave-assisted extraction method is a fast, efficient, energy saving and high-selective.

关 键 词：贯叶连翘 微波提取 总黄酮 正交试验 

分 类 号：R284.2[医药卫生—中药学]