牛蒡寡糖-钕配合物对西瓜枯萎病菌(Fusarium oxysporum f. sp. niveum)的影响  

Effect of Burdock Oligosaccharide-neodymium Complex on Fusaraum oxysporum f. sp. niveum

作　　者：张玉凤[1] 董亮[1] 杨力[1] 徐建玲[2] 

机构地区：[1]山东省农业科学院土壤肥料研究所农业部新型肥料创制重点开放实验室山东省肥料工程技术研究中心,山东济南250100 [2]山东省沂南县农业局,山东沂南276300

出　　处：《安徽农业科学》2009年第14期6472-6475,共4页Journal of Anhui Agricultural Sciences

基　　金：十一五国家科技支撑计划重点项目(2006BDA07A13-1-2);山东省农业科学院博士基金(2006YBS023);金大地研究院科研基金资助项目

摘　　要：[目的]确定牛蒡寡糖－钕配合物对病原菌的最佳作用浓度。[方法] 采用平板培养和液体培养法，研究了不同浓度牛蒡寡糖－钕配合物对尖镰孢菌菌落直径、果胶酶活性、菌丝生长量和菌丝细胞膜透性的影响。[结果] 在平板培养试验中，2～180 mg/L的牛蒡寡糖－钕配合物促进菌落扩展，但菌丝稀疏，200～400 mg/L 的牛蒡寡糖－钕配合物抑制菌落扩展，菌丝稀而薄。在液体培养试验中，2～400 mg/L的牛蒡寡糖－钕配合物抑制菌丝生长，抑制率为4.3%～64.4%，随着牛蒡寡糖－钕配合物浓度的升高，其抑制作用加强；2～400 mg/L 的牛蒡寡糖－钕配合物降低了果胶酶活性；当测定时间为15、30、60、90、120和180 min 时，2～400 mg/L的牛蒡寡糖－钕配合物增加了菌丝细胞膜透性。[结论] 该研究为牛蒡寡糖-钕配合物在植物上的合理应用提供了理论依据。[ Objective ] The research aimed to ascertain the optimum action concn, of burdock oligosaccharide-neodymium complex （Bo-Nd） on pathogens. [Method] The effects of Bo-Nd at different concn, onthe colony diameter, pectinase activity, yield and membrane permeability of mycelia of Fusaraum oxysporum f. sp. niveum were researched by plate culture and liquid culture methods. [ Result ] In plate culture experiment, the expansion of colony was promoted by Bo-Nd at 2 - 180 mg/L, but its mycelia were rare; the expansion of colony was inhibited by Bo-Nd at 200 - 400 mg/L and its mycelia were rare and thin. In liquid culture experiment, the growth of mycelium was inhibited by Bo-Nd at 2 -400 mg/L and its inhibitory rate was 4.3% -64.4%, as the concn, of Bo-Nd was increased, its inhibitory action was intensified. the pectinase activity was reduced by Bo-Nd at 2 -400 mg/L; when the detection period was 15, 30, 60, 90, 120 and 180 min, the membrane permeability of myeelia was increased by Bo-Nd at 2 -400 mg/L. [ Conclusion] The research supplied theoretical foundation for the reasonable application of Bo-Nd in plants.

关 键 词：尖镰孢菌 牛蒡寡糖-钕配合物 菌丝生长量 菌落直径 果胶酶活性 

分 类 号：S436.429[农业科学—农业昆虫与害虫防治]