杨树叶片蛋白质组分析  

The preliminary analysis of poplar leaf proteome

作　　者：袁坤[1] 潘惠新[2] 杨礼富[1] 王真辉[1] 王明庥[2] 黄敏仁[2] 

机构地区：[1]中国热带农业科学院橡胶研究所,海南儋州571737 [2]南京林业大学杨树研究开发中心,江苏南京210037

出　　处：《南京林业大学学报（自然科学版）》2009年第3期13-16,共4页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金：国家“十一五”科技支撑计划(2006BAD01A1503)

摘　　要：从杨树叶片的双向电泳蛋白图谱上随机选取12个蛋白点,经电喷雾串联质谱(Electrospray ionization tandemmass spectrometry,ESI-MS-MS)分析。结果显示,这12个蛋白点代表了5种不同的蛋白,其中包括:核酮糖二磷酸缩化酶/加氧酶(RuBisCO)、富甘氨酸RNA结合蛋白(Glycine-rich RNA-binding protein)、微管蛋白(alpha-tubulin)、叶绿体磷酸甘油酸激酶(chloroplast phosphoglycerate kinase)以及光敏色素(phytochrome B2)蛋白等。Twelve protein spots were randomly selected from poplar leaf protein profiles of two-dimension gel electrophoresis. By ESI - MS- MS analysis, the results showed that they represented five different proteins, including RuBisCO, Glycine-rich RNA-binding protein, alpha-tubulin, chloroplast phosphoglyeerate kinase and phytochrome B2. This study would lay the foundation for analysis of the whole proteome of poplar leaf and for the other biological research.

关 键 词：杨树叶片 ESI—MS—MS 蛋白质组 

分 类 号：S722[农业科学—林木遗传育种]