大鼠肾脏缺血再灌注损伤中垂体中叶素及降钙素受体样受体的表达  

Expression changes of intermedin and calcitonin receptor-like receptor in the kidney of rats after renal ischemia reperfusion injury

作　　者：于桂花[1] 李荣山[1] 乔晞[1] 周芸[1] 寇敏[1] 王晨[1] 白波[1] 邵珊[1] 

机构地区：[1]山西医科大学第二医院肾内科,太原030001

出　　处：《中华肾脏病杂志》2009年第5期387-391,共5页Chinese Journal of Nephrology

基　　金：国家自然科学基金（30771004）;山西省科技攻关项目（20080311061-6）;山西省高校科技研究开发项目（200811071）;山西医科大学博士启动基金（200625）;山西医科大学第二医院博士启动基金（2006-08）

摘　　要：目的观察垂体中叶素（IMD）及其受体降钙素受体样受体（CRLR）在大鼠肾脏缺血再灌注损伤中的表达变化。方法将健康雄性Wistar大鼠随机分为假手术组和手术组，夹闭大鼠双侧肾动脉制作肾脏缺血再灌注损伤（IRI）模型，于缺血再灌注后0、6、12、24、48、72h6个时间点各取6只大鼠，留取血清及肾组织标本，对肾脏病理损伤评分并行半定量分析；Western印迹法半定量分析肾组织IMD及其受体CRLR的表达变化；放射免疫法检测血浆中IMD的表达变化。结果手术组大鼠发生了急性肾小管坏死（ATN），以缺血再灌注48h时病理损伤最重。与假手术组比较，IMD及CRLR在缺血再灌注12、24、48、72h表达显著增高（均P〈0．01）；血浆中IMD在缺血再灌注12、48、72h表达显著增高（均P〈0．05）。结论IMD及受体CRLR在大鼠肾脏缺血再灌注损伤中表达增加，血浆中IMD表达上调，提示其可能在肾脏缺血再灌注损伤病理生理过程中发挥作用。Objective To investigate the expressions of intermedin /adrenomedullin 2 （IMD/AM2） and its receptor ealcitonin receptor-like receptor （CRLR） in the kidney of rats after renal ischemia reperfusion injury（IRI）. Methods Male Wistar rats were divided randomly into two groups： sham group and operation group. Renal IRI model was induced by clamping both renal arteries. Blood and kidney were harversted at 0 h, 6 h, 12 h, 24 h, 48 h, 72 h after reperfusion, respectively. Renal histological changes were semi-quantitated. Expressions of IMD and CRLR in the kidney were detected by Western blot, and the content of IMD in serum was measured by radioimmunity at 12 h, 48 h, 72 h after reperfusion. Results Kidneys of renal IRI model rats displayed significant pathologic changes, and the changes were much severer at 48 h after reperfusion. The expressions of IMD and CRLR in kidney were significantly up-regulated at 12 h, 48 h, 72 h after renal IRI （P〈0.01）. The level of IMD in serum increased at 12 h, 48 h, 72 h after renal IRI （P〈0.05）. Conclusion The expressions of IMD and its receptor are upregulated in the kidney after renal IRI, which may participate in the pathophysiological changes induced by renal IRI.

关 键 词：肾上腺髓质素 受体 降钙素 再灌注损伤 肾小管坏死 急性 

分 类 号：R692[医药卫生—泌尿科学] R-332[医药卫生—外科学]