同步扫描-双波长荧光分光光度法同时测定三种儿茶酚胺类神经递质  

Simultaneous Determination of 3 Catecholamine Neurotransmitters by Synchronous Scanning-Dual Wavelength Fluorospectrophotometry

作　　者：赵燕燕[1] 苏芳[2] 王翠玲[2] 姜明明[2] 白洁[1] 

机构地区：[1]河北大学医学实验中心,保定071000 [2]河北大学药学院,保定071002

出　　处：《理化检验（化学分册）》2009年第5期497-500,共4页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：河北省科技攻关资助项目(05276101D-88;42761220);河北省中医药管理局资助项目(05015);河北大学人才引进项目(y2004039);河北大学自然科学基金项目(2005408)资助

摘　　要：提出了用同步扫描-双波长荧光分光光度法同时测定肾上腺素(EP)、去甲肾上腺素(NEP)和多巴胺(DA)3种儿茶酚胺类神经递质。试验表明:荧光检测宜选定发射波长(eλm)与激发波长(eλx)的波长差为70 nm(Δλ=eλm-eλx)的条件下进行同步扫描。在eλm为385.0 nm时DA的荧光信号不受EP和NEP的干扰,而EP和NEP相互的干扰,采用双波长荧光检测模式可消除。选择测定NEP的波长对为470.0 nm(eλm,1)和531.8 nm(eλm,2),测定EP的波长对为500.0 nm(λ′em,1)和445.6 nm(λ′em,2)。测得荧光强度与3种儿茶酚胺浓度在320μg.L-1(EP),640μg.L-1(NEP)及160μg.L-1(DA)内呈线性关系,检出限(3σ/k)依次为0.20,0.97,0.73μg.L-1。Synchronous scanning DW-fluorospectrophotometry was proposed for the simultaneous determination of 3 catecholamine neurotransmitters, i. e. , epinephrine （EP） norepinephrine （NEP） and dopamine （DA）. It was found that satisfactory results were obtained by setting the wavelength difference （△λ） between emission wavelength （λem） and excitation wavelength （λex） at 70 nm in the synchronous scanning fluorescence measurements. The fluorescence signals of DA measured at the λem of 385. 0 nm were not interfered by EP and NEP, and the co-interference between EP and NEP was satisfactorily eliminated by adopting the mode of dual wavelength measurement. Hence, EP, NEP and DA were determined simultaneously in the same sample solution. The wavelength pairs of 470. 0 nm （λem.1） and 531.8 nm （λem.2） as well as 500. 0 nm （λ＇em,1） and 445.6 nm （λem,2） were chosen for the determination of NEP and EP respectively. Linear relationships between values of fluorescence intensity and concentration of the catecholamines were found in the ranges within 320 μg·L^-1 （for EP）, 640 μg·L^-1 （for NEP） and 160μg·L^-1 （for DA）, and the detection limits （3σ/k） found were 0. 20, 0. 97, 0. 73μg·L^-1 respectively.

关 键 词：同步扫描 双波长-荧光分光光度法 儿茶酚胺类神经递质 

分 类 号：O657.32[理学—分析化学]