^(18)F-FHBG PET裸鼠移植瘤的HSV1-tk基因显像  

^(18)F-FHBG PET imaging of HSV1-tk reporter gene expression in tumor xenografts of nude mice

作　　者：王全师[1] 李洪生[1] 唐刚华[1] 理东丽[1] 王巧愚[1] 吴湖炳[1] 

机构地区：[1]南方医科大学南方医院PET中心,广州510515

出　　处：《核技术》2009年第5期379-382,共4页Nuclear Techniques

基　　金：国家自然科学基金(30370426;30870731)资助

摘　　要：制备了18F-FHBG基因探针,构建表达HSV1-tk报告基因的逆转录病毒表达载体pLXSN-tk,并建立人肝癌BEL-7402裸鼠双侧腋窝稍靠背侧皮下移植瘤模型。右侧移植瘤为实验组,左侧移植瘤为对照组,将逆转录病毒转染实验组移植瘤细胞。经裸鼠尾静脉注射18F-FHBG后,于10、30、50、70、90、110、130、150、170、190、210min进行PET显像。结果显示:18F-FHBG未校正放化产率为4%-10%,经放射性HPLC分析测定放化纯度>95%。对照组移植瘤放射性分布未见明显增高;实验组移植瘤于注射18F-FHBG后50-210min,PET显像见到灰阶可分辨放射性浓聚,150min前摄取值逐渐升高,150min达到高峰(摄取值为19.772kBq·mL-1),150min后摄取值快速降低。实验组移植瘤的摄取值明显高于对照组移植瘤,两组移植瘤摄取值比值达4.99。表明18F-FHBGPET报告基因显像系统可用于监测HSV1-tk基因活体内靶细胞内的表达。Radiopharmaceutical of ^18F-FHBG was synthesized and recombinant expression plasmid pLXSN-tk was constructed. The nude mice sub-skin model transplanted with human liver cancer was constructed by subcutaneous implantation of cultured the BEL-7402 liver carcinoma cell lines into the two sides axillary fossa nude mice. The left tumors were used as the control, while the right tumors were used as the experiment group, with the cancer cell being transfected by the pLXSN-tk. PET images were taken at 10, 30, 50, 70, 90, 110, 130, 150, 170, 190 and 210 min after ^18F-FHBG injection via the tail veins. The results show that the uncorrected radiochemical yield of ^18F-FHBG was 4%-10% and the radiochemical purity was over 95%. The ^18F-FHBG PET images indicated that ^18F-FHBG uptake of the control did not increase obviously, whereas high activity was observed in the experiment group 50-210 min after ^18F-FHBG injection, ^18F-FHBG uptake of the experiment group increased gradually to 19.772 kBq·mL^-1 150 min after ^18F-FHBG injection, where the experiment/control tumor ratio was peaked at 4.99. The results indicated that ^18F-FHBG PET imaging of HSV1-tk expression in tumors can be used to monitor, in living animals, and expression of theoretical gene.

关 键 词：裸鼠 移植瘤 体层摄影术 发射型计算机 HSV1-TK 18F-FHBG 

分 类 号：R445.5[医药卫生—影像医学与核医学]