缺血性股骨头坏死患者血清同型半胱氨酸与超敏C反应蛋白的临床研究  

Study on serum homocysteine and high-sensitive C-reactive protein in patients with avascular necrosis in femoral head

作　　者：万革 袁新[2] 

机构地区：[1]中山医院骨外科,重庆市400014 [2]重庆医科大学附属第一医院骨科,400016

出　　处：《实用医学杂志》2009年第9期1415-1417,共3页The Journal of Practical Medicine

基　　金：重庆市自然科学基金资助项目(编号:cqsf07k012)

摘　　要：目的:检测缺血性股骨头坏死患者血清同型半胱氨酸(HCY)、超敏C反应蛋白(hs-CRP)、白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)浓度值,探讨HCY、hs-CRP在缺血性股骨头坏死发病机制中作用。方法:实验分为4组,非股骨头坏死正常组(N组)、股骨头坏死Ⅰ期组(N1组)、股骨头坏死Ⅱ期组(N2组)、股骨头坏死Ⅲ期组(N3组)。双抗体夹心ELISA法测定hs-CRP、IL-1β、TNF-α,采用荧光标记免疫检测法检测血清HCY水平,生物化学法测定血脂。结果:N1、N2、N3组和N组比较,HCY、hs-CRP、IL-1β、TNF-α浓度明显升高(P<0.01);N3组和N1组比较,HCY、hs-CRP、IL-1β、TNF-α浓度明显增高(P<0.01)。结论:HCY、hs-CRP参与缺血性股骨头坏死的发病机制。HCY参与缺血性股骨头坏死发生发展的炎性反应过程,炎症反应可能是缺血性股骨头坏死的促发因素。Objective To detect the serum levels of homocysteine （HCY）, high-sensitive C-reactive protein （hs- CRP）, interleukin-1β （IL-1β）, and tumor necrosis factor-α （TNF-α） in patients with avascular necrosis in femoral head, and to explore the role of HCY and hs-CRP in the pathogenesis of it. Methods Patients with avaseular necrosis in femoral head were divided into 4 groups, normal group （group N）, stage-one of necrosis in femoral head group （group N1 ）, stage- two of necrosis in femoral head group （group N2）, and stage-three of necrosis in femoral head group （group N3）. Serum levels of hs-CRP, interleukin-1β （IL-1β）, and TNF-α were measured by enzyme linked immunosorbent assay, HCY was measured by immunofluorescenee, and blood lipid was detected by biochemical method. Results The serum levels of HCY, hs-CRP, IL-1β, TNF-α were significantly higher in group N1, N2, and N3 than in group N （P 〈 0.01）, and were significantly higher in group N3 than in group N1 （P 〈 0.01）. Conclusions HCY and hs-CRP may participate in the pathogenesis of avascular necrosis in femoral head. HCY may play an important role in the process of inflammatory reaction, which may be a precipitating factor in avascular necrosis in femoral head.

关 键 词：股骨头坏死 同型半胱氨酸 超敏C反应蛋白 

分 类 号：R681.8[医药卫生—骨科学] R714.246[医药卫生—外科学]