急性炎症对黑色素瘤基质金属蛋白酶影响的研究  

Study on the Molecular Mechanism of Acute Inflammation in Suppressing MMPs in Melanoma

作　　者：马跃美[1,2] 赵秀兰[3] 孙涛[3] 赵楠[3] 古强[3] 倪春生[3] 郄硕[3] 刘祎[3,2] 孙保存[1] 

机构地区：[1]天津医科大学肿瘤研究所,300060 [2]天津医科大学手术外科学教研室 [3]天津医科大学病理学教研室

出　　处：《天津医药》2009年第5期380-382,I0003,共4页Tianjin Medical Journal

基　　金：国家自然科学基金资助项目(项目编号:30770828)

摘　　要：目的:研究急性炎症对黑色素瘤生长及肿瘤基质金属蛋白酶(MMP)-9和MMP-2的表达水平的影响。方法:采用C57BL小鼠,构建B16小鼠黑色素瘤动物模型,待肿瘤生长至0.5cm3时构建伤口模型,分为单纯肿瘤组(对照组)和伤口+肿瘤组(实验组),比较肿瘤体积大小,免疫组织化学染色检测肿瘤组织MMP-2和MMP-9的表达,明胶酶谱法分析MMP-2和MMP-9活性。结果:(1)实验组肿瘤体积在5d和7d时明显小于对照组(P<0.05或P<0.01)。(2)在造模后7d实验组MMP-2的细胞阳性表达率低于对照组(P<0.01),11d时2组比较差异无统计学意义(P>0.05);7d和11d实验组MMP-9阳性表达率均低于对照组(P<0.01)。实验组MMP-2和MMP-9酶活性在7d时低于对照组(P<0.01),11d时2组比较差异无统计学意义(P>0.05)。结论:急性炎症早期可影响肿瘤组织MMPs的表达,明显抑制黑色素瘤生长;而急性炎症晚期则对黑色素瘤生长和MMPs失去抑制作用。Objective： To investigate the effect of acute inflammation on melanoma growth and expression of matrix metalloproteinases-2 （MMP-2） and MMP-9. Methods： The B16F10 melanoma cell suspension （0.2 mL） was injected into the left groin area of C57BL mouse. The wound measured 3 cm long was built on the opposite side of bodies in the wound group. The control group was the C57BL mice without wound. The expressions of MMP-9 and MMP-2 were examined by immunohistochemistry staining and Gelatin Zymography. Results： The average tumor volume was significantly smaller at the 5^th d and 7^th d in wound group compared with that of control group （P 〈 0.05 or P〈 0.01）. The positive expression ratio of MMP- 2 was statistically higher at the 7^th d in wound group than that of the control group （P 〈 0.01）, but no significant difference at 11^th d between two groups. The positive expression of MMP-9 was higher at the 7^th d and 11^th d in wound group compared with that of the control （P 〈 0.01）. The enzyme activities of MMP-2 and MMP-9 were significantly higher at the 7^th d in wound group compared that of the control group （P 〈 0.01）, but no significant difference at the 11th d between two groups. Conclusion： The expression of MMPs may be inhibited at the early stage of acute inflammation, which ultimately inhibited the growth of B 16 melanoma.This inhibitory effect disappeared in tile later stage of acute inflammation.

关 键 词：黑色素瘤 炎症 基质金属蛋白酶类 急性病 

分 类 号：R979.1[医药卫生—药品] R730.231[医药卫生—药学]