泛素结合酶基因UbcH10在脑星形胶质细胞瘤组织中的表达及意义  

Expression of ubiquitin conjugating enzyme UbcHlO in brain astrocytomas

作　　者：蒋磊[1] 骆纯[1] 胡国汉[1] 黄承光[1] 陈菊祥[1] 孙克华[1] 卢亦成[1] 

机构地区：[1]第二军医大学长征医院神经外科,上海市神经外科研究所,上海210003

出　　处：《中华神经医学杂志》2009年第5期459-463,共5页Chinese Journal of Neuromedicine

基　　金：国家863计划引导项目（2005AA001070）

摘　　要：目的探讨泛素结合酶基因UbcH10在脑星形胶质细胞瘤组织中mRNA和蛋白表达水平及其与星形胶质细胞瘤病理级别的相关性。方法应用实时荧光定量PCR、免疫组织化学染色和蛋白印迹实验检测UbeH10 mRNA和蛋自在32例不同级别脑星形胶质细胞瘤和6例正常脑组织中的表达．并检测UbcH10蛋白表达与增殖抗原Ki-67蛋白表达的相关性。结果实时荧光定量PCR结果、免疫组织化学染色结果和蛋白印迹实验结果提示：与正常脑组织（1．00±1．57；0％；0．43±0．42）比较．UbcHlomRNA和蛋白表达水平在高级别星形胶质细胞瘤（64．33±60．98；9．65％±5．75％；0．69±0．38）和低级别星形胶质细胞瘤（8．36±8．15；4．82％±3．30％；0．10±0．08）中明显上调，差异均有统计学意义（P〈0．05）；且高、低级别星形胶质细胞瘤之间比较差异也有统计学意义（P〈0．05）。UbcH10阳性细胞表达率与增殖抗原Ki-67阳性细胞表达率呈显著的正相关关系（r=0．67，P=0．000）。结论泛素结合酶基因UbcH10可能在星形胶质细胞瘤的发生发展恶变过程中具有重要作用。Objective To investigate the expression ofubiquitin conjugating enzyme UbcHlO gene at mRNA and protein levels in brain astrocytomas and its correlation to the pathological grades of astrocytomas. Methods Quantitative real-time RT-PCR analysis, immunohistochemistry and Western blot were performed to detect the expression of UbcHlO mRNA and protein in 32 astrocytomas of different pathological grades and 6 normal brain tissues. The correlation between UbcH10 and Ki-67 immunoreactivity was examined with the Spearman＇s correlation coefficient. Results Statistical analysis showed significantly increased expression levels of UbeHIO mRNA in high-grade astrocytomas （64.33±60.98） in comparison with those in low-grade astrocytomas （8.36±8.15） and normal brain tissues （1.00±1.57） （P〈0.05）. Increased UbcH10 labeling index in immunohistochemistry was also noted in high-grade astrocytomas [（9.65±5.75）%] as compared with that in low-grade astrocytomas [（4.82± 3.30）%] （P〈0.05） and normal brain tissues [0%, P〈0.05）. Western blot demonstrated significantly higher UbcH10 protein levels in high-grade astrocytomas （0.69±0.38） than in low-grade astrocytomas （0.10±0.08, P〈 0.05） and normal brain tissues （0.01±0.02, P〈 0.05）. UbcH 10 positivity rate was found to positively correlate to that of Ki-67 （r-=0.67, P=-0.000）. Conclusion Overexpression of UbcH10 may play an important role in the development and progression of astrocytomas.

关 键 词：神经胶质瘤 星形胶质细胞瘤 泛素结合酶基因UbcH10 增殖抗原Ki-67蛋白 

分 类 号：R686[医药卫生—骨科学]