Kir4．1和AQP4在大鼠局灶性脑缺血再灌注损伤中的作用  

Role of Kir4.1 and aquaporin-4 in focal brain ischemia-reperfusion injury in rats

作　　者：孙伟[1] 苏志强[1] 刘建丰[1] 盛利[1] 杨鲲鹏[1] 赵静奕[2] 吴向阳[2] 

机构地区：[1]哈尔滨医科大学附属第一医院神经内科,哈尔滨150001 [2]武汉市空军雷达学院院务部卫生处,武汉430019

出　　处：《中华神经医学杂志》2009年第5期484-487,共4页Chinese Journal of Neuromedicine

摘　　要：目的观察大鼠局灶性脑缺血再灌注模型Kir4．1和AQP4的变化，探讨其在再灌注损伤中的作用。方法雄性Wistar大鼠50只，按照随机数字表法分成假手术组、缺血2h再灌注3h组、12h组、24h组和72h组，每组10只。应用“线栓法”实现大鼠右侧大脑中动脉闭塞．2h后拔出线栓进行再灌注，并在相应时间点处死大鼠。利用免疫组化和实时定量PCR（RT—PCR）法观察Kir4．1和AOP4蛋白表达及mRNA水平的变化。结果与假手术组相比，缺血再灌注组大鼠梗死灶周围区皮质Kir4．1、AOP4的蛋白表达和mRNA水平明显升高，于再灌注后3h开始升高，24h达到高峰．72h开始下降。假手术组、缺血再灌注3h、12h、24h和72h组Kir4．1mRNA水平分别为0．34±0．02、0．47±0．06、0．61±0．08、0．83±O．10、O．68±0．09，AOP4的mRNA水平分别为0．49±O．05、0．66±0．09、0．91±0．09、1．12±0．11、0．94±0．08。Kir4．1与AOP4的mRNA水平呈正相关（r=0．780，P=-0．000）。结论Kir4．1和AQP4相互作用，共同参与了脑缺血再灌注损伤的形成．Objective To observe the expressions of Kir4.1 and aquaporin-4 （AQP4） in rats with focal brain isehemia-reperfusion （IR） injury and clarify the role of Kir4.1 and AQP4 in IR injury. Methods Male Wistar rats were randomized into 5 groups, including a sham-operated group and 4 IR groups with focal brain IR injury induced by middle cerebral artery occlusion （MCAO）. In the 4 IR groups, the rats were subjected to a 2-hour ischemia and sacrificed after reperfusion for 3, 12, 24 or 72 h. Immunohistochemistry and RT-PCR were used to detect the expressions of Kir4.1 and AQP4 proteins and mRNAs in the brain tissues, respectively. Results Compared with the sham-operated group, the IR groups showed significantly increased expressions of Kir4.1 and AQP4 in the peri-infarct cortex surrounding the primary infarct area （P〈0.05）, which began at 3 h after the reperfusion and reached the peak level at 24 h, subsiding at 72 h. The mRNA levels of Kir4.1 （represented as the absorbance） were 0.34±0.02, 0.47±0.06, 0.61±0.08, 0.83±0.10 and 0.68±0.09 in the sham-operated group and the 4 IR groups with reperfusion for 3, 12, 24 and 72 h, respectively. The mRNA levels of AQP4 were 0.49±0.05, 0.66±0.09, 0.91±0.09, 1.12±0.11 and 0.94±0.08 in the 5 groups, respectively. Kit4.1 expression was positively correlated to AQP4 expression at the mRNA level （r=0.780, P=0.000）. Conclusion Kir4.1 and AQP4 react with each other in the course of focal brain ischemia-reperfusion, both playing roles in the mechanism of brain IR injury.

关 键 词：脑缺血 再灌注损伤 内部整流钾离子通道4.1 水通道蛋白 

分 类 号：R686[医药卫生—骨科学]