猪瘟病毒与猪流行性乙型脑炎病毒复合RT-PCR检测方法的建立  

Multiplex RT-PCR for Detection of Classical Swine Fever Virus and Japanese Encephalitis Virus

作　　者：薛媛[1] 杨柳絮[1] 张淼涛[1] 

机构地区：[1]西北农林科技大学动物医学院,陕西杨凌712100

出　　处：《西北农业学报》2009年第3期14-17,共4页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：陕西省自然科学基金项目(2005C105)

摘　　要：猪瘟病毒(CSFV)和流行性乙型脑炎病毒(JEV)是引起种猪繁殖障碍的病原,而且经常混合感染,及时准确诊断是防治的前提。根据CSFV的E2基因保守序列和JEV的E基因保守序列,选取2对引物建立检测CSFV、JEV的复合RT-PCR方法,分别扩增出了大小为508和1 015 bp特异性基因片段。应用此方法检测了10份临床样品(淋巴结、脾、肝、肺等组织),结果4份CSFV阳性,3份JEV阳性,其余为阴性。结果表明此CSFV和JEV复合RT-PCR诊断方法,具有很好的特异性和敏感性,可用于临床检测。Classical swine fever virus （CSFV） and Japanese encephalitis virus （JEV） are the major causes of some serious reproductive diseases and co-infection is common. Therefore rapid and reliable diagnosis is crucial for effective prevention and treatment. In this study, a multiplex RT-PCR was developed using two-pairs primers designed according to conserved sequences in E2 fragments of CSFV and that in E fragments of JEV. PCR products of 508bp and 1015bp were obtained for respective viruses. Ten organ samples （lung, lumph, liver, spleen, etal） were respectively detected by the multiplex RT-PCR,out of which four were CSFV positive, three were JEV positive , the control samples were negative. The results showed that the multiplex RT-PCR of CSFV and JEV was a rapidly specific and sensitive diagnostic method.

关 键 词：复合RT-PCR CSFV JEV 

分 类 号：S858.28[农业科学—临床兽医学]