不同浓度的EGF对山羊毛囊干细胞体外培养的影响  

Effect of Different Concentrations of EGF on the Culture of Goat Hair Follicle Stem Cells

作　　者：史明艳[1,2] 林峰[3] 杨学义[1] 窦忠英[2] 

机构地区：[1]洛阳师范学院生命科学系,河南洛阳471022 [2]西北农林科技大学国家干细胞工程技术研究中心陕西分中心,陕西杨凌712100 [3]河南农业大学畜牧兽医工程学院,河南郑州450002

出　　处：《西北农业学报》2009年第3期26-28,共3页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家自然科学基金(No.30200137);国家“863”计划(No.2002AA216161);教育部重大科技专项(03160)

摘　　要：采用2.4 U/mL Dispase酶消化和机械切割法分离毛囊隆突部,经胰酶(0.5 mg/mL胰酶+0.2 mg/mL EDTA)消化从山羊耳部皮肤分离得到毛囊干细胞,并检测4种不同浓度(0、10、15和20 ng/mL)的EGF对山羊毛囊干细胞体外培养的作用。结果表明,EGF对毛囊干细胞体外培养效果明显,最适EGF浓度为15ng/mL。Hair follicles were isolated from goat ear skin by were separated with special fine needle and digested with O. The resultant cells were cultured in four different concentr results showed that EGF has a good effect on the goat hair was best congcentration. 2.4U/mL Dispase digestion and the bulges 5 mg/mL trypsin plus 0.2 mg/mL EDTA. ations of EGF（0,10 ,15 ,20 ng/mL）. The follicle stem cells in vitro and thel5 ng/mL

关 键 词：毛囊干细胞 克隆形成率 EGF 体外培养 

分 类 号：S827[农业科学—畜牧学]