淡紫拟青霉PL04菌株抗逆性及其对香蕉根结线虫的寄生作用  

Stress Resistance Characteristics of the PL04 Strain of Paecilomyces lilacinus and Parasitism Effect of It on Meloidogyne spp.

作　　者：汪军[1] 黄俊生[1] 邓国平[1] 赵培静[1] 杨腊英[1] 陈希德[1] 

机构地区：[1]中国热带农业科学院环境与植物保护研究所,海南儋州571737

出　　处：《西北农业学报》2009年第3期263-267,共5页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家科技支撑计划项目(2007BAD48B02);中央级公益性科研m院所基本科研业务费专项(2007HZS1J007);科技部"国家科技基础条件平台工作"(2005DKA21201);农业科技成果转化项目(2007GB23260410)资助

摘　　要：抗逆性测定结果表明，淡紫拟青霉PL04菌株有较强的抗逆性和对化学农药的兼容性。PL04菌株在PSA培养基上菌落生长和产孢的适温为5-40℃（最适温度为29℃）。PL04菌株在pH4-13（最适pH为7）范围内的培养基上均可生长；PL04菌株在40℃和50℃条件下的LT50分别为78．8min和40．8min。PL04菌株有较强的耐盐碱能力；PL04菌株对氯霉素有较强的耐受性；PL04菌株对杀菌剂多菌灵、百菌清、苯醚甲环唑、除草剂百草枯以及消毒防腐剂福尔马林较敏感，其EC50分别为0．021、0．013、0．006、0．016和0．120mg／mL，PL04菌株对杀线虫剂阿维菌素和辛硫磷，杀菌剂三唑酮不敏感，其EC。。分别为0．175、0．338和0．337mg／mL。采用双温测定法，室内测定淡紫拟青霉PL04菌株对香蕉根结线虫的寄生作用，不同处理具有显著性差异，PL04菌株孢子悬浮液处理18d后对香蕉根结线虫卵的相对寄生率为94．3％。Stress resistance characteristics tests showed that the strain PL04 strain of Paecilomyces li- lacinus had strong resistance ability and was compatibility with chemistry pesticide. For the growth and spore production of PL04 strain, the suitable temperature was 5-40 °C （the optimum temperature was 29°C）. The PL04 strain could grow on potato sugar agar with pH range from 4 to13（the optimum pH was 7）. Tests showed that the LT50 at 40°C was 78.8 min and at 50°C was 45.3 min of the PL04 strain. The PL04 strain was able to endure higher concentration saline-alkali and chloramphenicol. Tests showed that the PL04 strain was sensitive to fungicide carbendazol, chlororthalonil, difenoconazole, herbicide paraquat and formalin, EC50 of them were 0. 021 mg/mL, 0. 013 mg/mL, 0. 006rag mg/mL, 0. 016 mg/mL and 0. 120 mg/mL respectively. But the PL04 strain was consistent to the nemacicide avermecti, phoxim and fungicide trazodone, EC50 of them were 0. 175 mg/mL, 0. 338 mg/ mL and 0. 337 mg/mL respectively. The PL04 strain was demonstrated as a highly effective strain to control Meloidogyne spp. There was significantly difference among the different treatment. Results of the petridish test showed 94.3% of the eggs were parasitized by the fungus 18 d （7 days at 18°C plus 11 days at 25°C） after inoculation.

关 键 词：淡紫拟青霉 抗逆性 香蕉根结线虫 寄生作用 

分 类 号：S436.68[农业科学—农业昆虫与害虫防治]