瑞舒伐他汀对兔心肌缺血-再灌注损伤内皮功能的影响  

Effects of rosuvastatin on endothelial function during myocardial ischemia.reperfusion injury in rabbits MA

作　　者：马建群[1] 张蕾[1] 王连文[2] 朱玉琴[1] 张长元[1] 

机构地区：[1]山东省滨州医学院附属医院心内科,山东省滨州256603 [2]山东省滨州医学院附属医院核医学科,山东省滨州256603

出　　处：《中华急诊医学杂志》2009年第5期508-511,共4页Chinese Journal of Emergency Medicine

摘　　要：目的研究兔心肌缺血-再灌注损伤中内皮功能改变及瑞舒伐他汀对其影响。方法将16只新西兰大白兔随机分为两组：心肌缺血-再灌注损伤组（对照组），瑞舒伐他汀干预组（药物组）。建立心肌缺血-再灌注模型，心电图显示sT段明显弓背向上抬高（≥0．2mv）表示结扎左前降支成功；40min后剪断结扎线，心电图sT段回落1／2以上，标志再灌注成功。分别在缺血前，缺血40min，再灌注60min和再灌注180min四个时相点留取血标本，检测一氧化氮（nitric oxide，NO）、内皮素-1（endothe-lin-1，ET-1）含量。运用SPSS11．5软件，采用重复测量资料的方差分析，以P〈0．05为差异具有统计学意义。结果对照组与药物组血清NO含量随缺血及再灌注时间的延长而降低，血浆ET-1含量随缺血及再灌注时间的延长而增高；缺血前对照组与药物组血清NO含量[（109．875±32．255）μmol／L VS．（114．500±37．405）μmol／L，P〉0．05]及血浆ET-1含量[（221．111±28．125）pg／mL VS．（204．594±31．790）pg／mL，P〉0．05]差异无统计学意义；缺血40min、再灌注60min、再灌注180min时，药物组血清NO含量[分别为（63．125±18．962）、（43．500±16．518）、（29．625±14．162）μmol／L VS．（82．000±13．825）、（63．375±17．541）、（50．250±18．987）μmol／L，P〈0．05]均显著高于对照组，药物组血浆ET-1含量1分别为（331．785±35．341）、（375．914±45．204）、（459．829±70．110）pg／mL VS．（282．541±38．928）、（315．152±55．263）、（377．795±60．427）pg/mL，P〈0．05]均显著低于对照组。结论瑞舒伐他汀通过升高血清中NO，降低血浆ET-1．从而改善兔心肌缺血-再灌沣损伤时内皮功能。Objective To study the change of endothelial function during myocardial ischemia-repeffusion injury in rabbits and the effect of Rosuvastatin. Method Sixteen New Zealand rabbits were randomLy divided into two groups： ischemia/reperfusion injury group （control group） and Rosuvastatin group（drug group）. The myocar- dial ischemia-reperfusion model was established by occlusion of left anterior descending coronary artery for 40 min- utes evidenced by the elevation of the ST segment ≥ 0.2 mv on ECG waveform, and after release of occlusion, the ST segment of ECG returned to 1/2 or more of the normal waveform, which was the evidence of successful reperfu- sion. The rabbit serum nitric oxide （NO） and plasma endotbelia-1 （ET-1） content were assayed before occlusion, 40 minutes, 60 minutes and 180 minutes after reperfusion. SPSSll. 5 software was used for ANOVA（Repeated Measurement designs）. P 〈 0.05 was considered as statistically significant. Results There were no significant differences in serum NO and plasma ET-1 between two groups bsfore ischemia, but 40 minutes, 60 minutes and 180 minutes after reperfusion, the levels of serum NO in drug group were higher than those in control group [（82.000± 13.825）, （63.375 ± 17.541）, （50.250 ±18.987）μmol/L vs. （63.125± 18.962）, （43.500 ±16.518）, （29.625± 14.162） μmol/L, P 〈 0.05],and the levels of plasma ET-1 content in drug group were lower than those in the control group [ （282.541 ± 38.928）, （315.152 ±55.263）, （377.795 ± 60.427） pg/mL vs. （331.785±35.341）,（375.914±45.204）,（459.829±70.110） pg/mL, P 〈0.05]. Conclusions Bythe means of increasing serum NO and decreasing plasma ET-1, rosuvastatin can improve the endothelial function in rabbits with isehemia-reperfusion injury.

关 键 词：瑞舒伐他汀 心肌缺血-再灌注损伤 一氧化氮 内皮素-1 兔 

分 类 号：R96[医药卫生—药理学]