前列腺素E1预先给药对出血性休克复苏后大鼠肾组织Caspase-3表达的影响  

The effects of pretreatment with lipo-PGE1 on expression of Caspase-3 of kidney after hemorrhagic shock and resuscitation in rats

作　　者：何非方[1] 李利[2] 钟泰迪[1] 

机构地区：[1]浙江大学医学院附属邵逸大医院麻醉科、浙江大学邵逸火临床医学研究所,杭州310016 [2]浙江大学医学院附属第一医院ICU

出　　处：《中华急诊医学杂志》2009年第5期512-515,共4页Chinese Journal of Emergency Medicine

摘　　要：目的探讨PGE1预先给药对急性出血性休克复苏后大鼠肾组织活化Caspase-3以及TNF-α mRNA，iNOS mRNA表达的影响。方法32只雄性SD健康大鼠随机分成4组（n=8），A：正常对照组，不进行任何处理；B：手术埘照组，0．5mL生理盐水尾静脉注射，1h后切开左侧腹股沟皮肤暴露股动静脉；C：出血性休克复苏组，大鼠硫喷妥纳麻醉后，经股静脉穿刺置管放血，平均动脉血压降至25～35mmHg，维持1h，自体温血复苏30min，维持平均动脉血压80～100mmHg，制作失血性休克复苏模型；D：出血性休克复苏＋lipo-PGE1预先给约组，lipo-PGE1按10μg／kg溶于0．5mL生理盐水尾静脉注射，1h后制作出血性休克复苏模型。各组分别于复苏后6h时间 点用Northern blotting法检测肾组织TNF-α mRNA和iNOS mRNA的表达；免疫组化检测肾脏组织活化Caspase-3的表达。数据采用单因数方差分析（SNK-q检验），以P〈0．05为差异具有统计学意义。结果出血性休克复苏模型组肾组织出现Caspase-3阳性表达细胞；TNF-α mRNA和iNOS mRNA的表达也明显高于正常对照组[（0．651±0．028）VS．（0．030±0．003），（0．524±0．022）VS．（0．026±0．003），P〈0．05]和手术对照组[（0．651±0．028）VS．（0．029±0．002），（0．524±0．022）VS．（0．025±0．003），P〈0．05]；预先给药组肾组织Caspase-3阳性细胞数量显著减少；TNF-α mRNA和iNOS mRNA的表达明显低于模型组[（0．250±0．019）VS．（0．651±0．028），（0．203±0．020）VS．（0．524±0．022），P〈0．05]，正常对照组和手术对照组间。肾组织Caspase-3的表达以及TNF-α mRNA和iNOS mRNA的表达无显著差异[（0．029±0．002）vs．（0．030±0．003），（0．029±0．002）VS．（0．030±0．003），P〉0．05]。结论lipo-PGE1预先给药可以下调出血性休克复苏后大鼠肾组织Caspase-3的表达水平，其机制叮能与在基因转录水平抑制TObjective To observe the effects of pretreatment in rats with prostaglandin E1 on expression of kidney caspase-3, anti TNF-α mRNA and iNOS mRNA after hemorrhagic shock and resuscitation（HSR）. Method Totally 32 male S-D rats were randomly divided into four groups： group A （normal group, n = 8）, rats didn＇t receive any treatment; group B（sham, n = 8）, rats were pretreated with normal saline and underwent all experi- mentation procedures except bleeding; group C （HSR, n = 8） ： rats were anesthetized with intraperitoneal sodium pentobarbital（30 mg/kg） and then subjected to hemorrhagic shock followed by resuscitation; group D （lipo-PGE1 ＋ HSR, n = 8） ： rats were pretreated with lipo-PGE1 one hour before HSR. The expressions of TNF-α mRNA and iNOS mRNA was measured by Northern blotting analysis and the expression of caspase-3 at 6 hours was determined by immunohistoehemistry after HSR. Data were analyzed by ANOVA （SNK- q test）, and P 〈 0.05 was considered as significantly different. Results There were no differences in TNF-α mRNA and iNOS mRNA between normal group and sham group [（0.029±0.002）vs. （0.030±0.003）,（0.029±0.002） vs. （0.030±0.003）, P 〉 0.05]. HSR led to overexpression of easpase-3. The expression of kidney TNF-α mRNA anti iNOS mRNA also increased in HSR group, compared with normal group [ （0.651 ＋ 0.028） vs. （0.030 ± 0.003）, （0.524 ±0.022） vs. （0.006±0.003）, P 〈0.051 and sham group[（0.651 ±0.028） vs. （0.029±0.002）, （0.524±0.002） vs. （0.025 ± 0.003）, P 〈 0.05J. Pre-treatment with lipo-PGE1 markedly reduced the expression of Caspase-3, which was consitent with decrease in expressions of TNF-α mRNA and iNOS rnRNA 6 hours after HSR[ （0.250 ±0.019） vs. （0.651±0.008）, （0.203±0.000）vs. （0.524±0.002）, P 〈0.050]. Conclusions Lipo-PGE1 could reduce the expressions of kidney Caspase-3 after HSR. The mechanisms might be attributed to inhibiting the expression of TNF-α mRNA and iNOS mRNA

关 键 词：肾脏 出血性休克复苏 前列腺素E1 Caspase-3 

分 类 号：R459.7[医药卫生—急诊医学]