利用卡那霉素筛选非洲菊转基因植株的技术研究  

Study on Selecting of Transgenic Gerbera Plants with kanamycin

作　　者：张宝琼[1,2] 范眸天[2] 屈云慧[1] 于丽霞[3] 李涵[1] 

机构地区：[1]云南省农科院花卉研究所,云南昆明650205 [2]云南农业大学,云南昆明650201 [3]西南林学院,云南昆明650224

出　　处：《安徽农学通报》2009年第3期39-40,34,共3页Anhui Agricultural Science Bulletin

基　　金：云南省攻关及高新技术发展计划项目(2006NG34);云南省科技计划项目2007C118M共同资助

摘　　要：为了能够更高效地得到非洲菊转基因植株,将非洲菊的植株接种于含有不同浓度卡那霉素的分化培养基中,当卡那霉素浓度达到25 mg/L时,外植体的生长分化完全受抑制,苗白化。将非洲菊通过愈伤组织诱导,农杆菌介导得到转基因植株,把共培养3 d的外植体进行3种不同处理。结果表明,处理3较好,即先在培养基中直接加羧苄霉素不加卡那霉素,待苗长势较佳时,再用卡那霉素筛选,PCR检测,得到7棵阳性植株,再生率为43.75%。既保证了芽诱导苗的成活率,又节省了大量提取DNA的时间和贵重药品的使用量。In order to gain more efficient Gerbera transgenic plants, Gerbera plants were inoculated with different concentra- tions of Km in the proliferation medium, The concention of Kanamycin at 25 mg/L completely inhibited the explant growth and not shoot differentiation , young plant whitening. Through the Gerbera callus induction, Agrobacterium - mediated method to be transgenic plants, the co - cultured materials a total of 3 - day explants for 3 to deal with in co - culture will be dis- seminated stage of the explant for 3 to deal with. The results show that a better disposal3, that is, the first increase in the me- dium directly Carbenicillin - carboplatin did not Kanamycin, seedling growth to be better at using kanamycin selection, pcr testing, getting 7 transgenic plants. 43.75 % regeneration rate. Ensured the bud induction rate, saving more time and material medica.

关 键 词：非洲菊 农杆菌 卡那霉素 

分 类 号：Q78[生物学—分子生物学]