卡那霉素对昆明小鼠听脑干反应和畸变产物耳声发射的影响  

Effects of Kanamycin on auditory brainstem response and distortion product otoacoustic emission in Kunming mice

作　　者：李昱[1] 王爱梅[1] 宝东艳[1] 常志杰[1] 

机构地区：[1]辽宁医学院生理学教研室,辽宁锦州121001

出　　处：《中国现代医学杂志》2009年第9期1339-1341,1345,共4页China Journal of Modern Medicine

基　　金：辽宁省教育厅科学研究计划资助(No:202173374);辽宁省博士科研启动基金(No:20031086)

摘　　要：目的研究卡那霉素(KM)对昆明小鼠听脑干反应(ABR)和畸变产物耳声发射(DPOAE)的影响,探讨昆明小鼠对氨基糖甙类抗生素(AmAn)的耳毒易感性。方法16只雄性昆明小鼠随机分为KM组和对照组,给于皮下注射KM[2×750mg/(kg·d)]或者等量生理盐水,连续用药2周,分别记录用药前、后ABR阈值和DPOAE幅值;应用硝酸银染色观察耳蜗形态学变化。结果KM组用药1周后,DPOAE幅值明显下降,而ABR阈移无显著变化;2周后ABR阈移显著增大且停药后进一步增大。对照组ABR阈移和DPOAE幅值在用药前后无明显改变;耳蜗硝酸银染色显示对照组外毛细胞排列整齐,结构完整,而KM组外毛细胞则发生大部分缺失。结论昆明小鼠对AmAn易感,应用KM可以建立昆明小鼠AmAn耳毒性模型。在听功能检测上DPOAE可能比ABR更灵敏。[Objective] To investigate the effects of Kanamyein （KM） on auditory brainstem responses （ABR） and distortion product otoaeoustic emission （DPOAE） in Kunming mice, and to explore the susceptibility of aminoglycoside antibiotics （AmAn） induced ototoxicity in Kunming mice. [Methods] 16 male Kunming mice were randomly divided into KM group and control group. Mice were injected subcutaneously with KM （2×750 mg/kg·day） or equal physiological saline for 2 weeks. Auditory thresholds and DPOAE amplitudes were recorded respectively before and after injections. Morphological changes of the cochlea were observed by silver nitrate staining. [Results] After treatment with KM for 1 week, DPOAE amplitudes were dramatically decreased, while auditory threshold shifts were not obvious changed. Threshold shifts were significantly elevated after 2 weeks of injections, and increased further after cessation of treatment. There were no remarkable changes of auditory threshold shifts and DPOAE amplitudes in the eontrel group. The silver nitrate staining of cochlea showed an orderly outline of outer hair cells in control group, while KM-treated animals exhibited severe to subtotal outer hair cells loss. [ Conclusion] Kunming mouse was susceptible to AmAn, and a Kunming mouse model of AmAn induced ototoxicity could be established by administration of KM. DPOAE might be more sensitive than ABR in auditory function detection.

关 键 词：卡那霉素 听脑干反应 畸变产物耳声发射 耳毒性 

分 类 号：R764.43[医药卫生—耳鼻咽喉科]