脱细胞角膜材料的生物相容性研究  

Experimental study on histocompatibility after xenogeneic acellular feline lamellar keratoplasty

作　　者：吴连胜[1,2] 陈建苏[1] 徐锦堂[1] 丁勇[3] 郑佩娥[4] 徐国风 黄慧研 

机构地区：[1]暨南大学医学院眼科研究室再生医学教育部重点实验室,广州510632 [2]东莞市凤岗镇雁田广济医院,523690 [3]暨南大学附属第一临床医学院眼科,广州510632 [4]暨南大学医学院病理教研室,广州510632 [5]广东冠昊生物科技有限公司,广州510632

出　　处：《眼科研究》2009年第5期359-363,共5页Chinese Ophthalmic Research

基　　金：国家自然科学基金(30371519);广东省科技计划项目(2006B35801003,2007B031001005);暨南大学侨办重点学科(51205004)资助

摘　　要：目的研究异种脱细胞猫角膜板层角膜移植术后的组织相容性。方法24只18周龄的健康新西兰白兔随机平均分成3组:A组为脱细胞猫角膜材料→兔板层移植组,B组为猫→兔异种新鲜角膜植片板层移植组,C组为兔→兔同种异体新鲜角膜植片板层移植组。用扫描电镜观察术后不同时期术眼角膜的组织病理学改变,抽取兔血测定CD4+/CD8+T细胞百分比。结果术后1个月3个组间角膜植片炎症反应指数组间比较差异均无统计学意义;2个月A组与B组、A组与C组间比较差异均有统计学意义(P<0.05)。3个月A组与B组、A组与C组间比较差异均有统计学意义(P<0.05)。3个组间术后同期7、15、30d兔外周血CD4+T细胞和CD8+T细胞活化率差异均无统计学意义(P>0.05)。组织切片显示术后3个月内,B、C两组角膜植片炎症反应轻,植片内皮细胞吸收,组织结构基本正常。A组角膜植片炎症反应显著,结构较紊乱,新生血管增生。扫描电镜可见B、C2组角膜上皮结构基本正常,细胞连接较好。A组扫描电镜可见有上皮缺损。结论脱细胞处理的异种猫角膜移植后生物相容性较新鲜角膜差,免疫反应无显著差异。Objective The present study was to explore the histoeompatibility, graft survival time and inflammatory response after xenogeneie acellular cat lamellar keratoplasty onto rabbits. Methods Twenty-four healthy 18-week-old male New Zealand white rabbits were as reeeiptors and randomly divided into three groups：group A received cat-rabbit acellular xenogeneie lamellar keratoplasty; group B underwent cat-rabbit xenogeneic lamellar keratoplasty; group C was rabbit-rabbit corneal allograft lamellar keratoplasty, The diameter of the donor graft was 9 mm and the diameter of receipt bed was 8 mm. The inflammatory response and survival of graft were observed for 3 months. Histological and ultrastructure examination was performed at the 1 st, 2nd and 3rd month under the optical microscope and scanning electron microscope, respectively, and the percentages of CD4^＋/CD8^＋ from rabbit periphery blood were detected at the 7th, 15th and 30th day after the surgery. Results The inflammation index was 2. 38±1. 506,1.75 ± 1. 488 and 1. 25 ± 1. 282 in the 1st month in group A,B and C without significant difference among three groups （ P 〉 0.05 ） , and that in the 2nd month was significantly different between group A and group B（5.83±1.722 vs2.50±1.517） or between group A and group C（5.83±1.722 vs 2. 50±1.378）（P〈0.05）.In the 3rd month,the inflammation index was remarkably higher in group A compared with group B and group C （6.50 ± 0. 774 vs 2. 50±1. 291,6.50±0. 774 vs 2.75±0. 957） （P 〈0. 05）. No significantly differences were found in activation rates of CD4 ＋ T cells and CD8 ＋ T cells upon surgery time and among different groups（P 〉 0. 05） based on two-way ANOVA. Histological sections showed that the inflammatory reaction was more slight in group B and group C than group A three months after operation. Corneal epithelial cells were stratificated and cellular boundary were tightly opposed,but epithelial defect was displayed in group A. Conclusion The bioeompatibility of the acell

关 键 词：角膜 脱细胞生物材料 板层角膜移植 异种角膜移植 免疫反应 

分 类 号：R318.08[医药卫生—生物医学工程]