牛蛙肝脂和体脂中脂肪酸的气相色谱分析及其多不饱和脂肪酸的提取  

The Analysis of Fatty Acid Composition of Rana Catesbeiana Liver and Adipose Tissues and Studies on the Purification of Polyunsaturated Fatty Acids

作　　者：徐秀兰[1] 胡磊军[1] 吴梧桐[1] 

机构地区：[1]中国药科大学生物制药学院,南京210009

出　　处：《药物生物技术》1998年第1期30-33,共4页Pharmaceutical Biotechnology

摘　　要：用毛细管气相色谱法和银化薄层色谱法对牛蛙肝脏及脂肪组织中的脂肪酸成分进行了分析 ,发现 α-亚麻酸在肝脏和脂肪中的含量分别达到 2 2 .84 %和 16.0 6% ,用低温金属盐沉淀法可除去大部分饱和脂肪酸 ,对含有两个及多双键的不饱和脂肪酸有浓缩作用 。The fatty acid composition of Rana Catesbeiana (bullfrog) liver and adipose tissues was examined by GC combined with Ag TLC. It was found that the content of α linolenic acid (18∶3,n 3) in both tissues was very high (adipose, 22.84%; liver,16.06%). They could be used as sources to extract that important fatty acid. The lipid fractionation characters of low temperature crystallization were also studied. It was found that this method could eliminate most saturated fatty acids, and could concentrate the content of polyunsaturated fatty acids having two or more double bonds. But its effect on monoenoic fatty acid was not prominent.

关 键 词：毛细管 气相色谱法 脂肪酸 肝脏 脂肪 牛蛙 

分 类 号：Q959.53[生物学—动物学] S966.31[农业科学—水产养殖]