漆酶对染料的脱色研究  

Study on Decolorization of Dyes Caused by Laccase

作　　者：朱启忠[1] 吕新萍[1] 徐国英[1] 艾良[1] 宋炳红[1] 李文静[1] 

机构地区：[1]山东大学威海分校海洋学院,山东威海264209

出　　处：《资源开发与市场》2009年第6期484-486,共3页Resource Development & Market

基　　金：山东大学威海分校科研资助课题(编号:A2008001)

摘　　要：利用来源于白毒鹅膏菌的粗漆酶与12种工业染料反应,结果发现其中7种染料颜色能被脱除。研究了直接影响黑G和中性黄GL的漆酶脱色的相关因素。试验发现,这两种染料在1h内与漆酶反应迅速,且特征颜色吸收峰(600nm/440nm)基本消失,其最适温度均为50℃、最适反应pH值范围分别为5—6和3—4,酶活力的增加可显著提高反应速率,但对总脱色率影响不大。在pH值为5、温度40℃条件下,5U/ml的漆酶与直接黑G和中性黄GL反应的Km值分别为3.2mg/L和25.1mg/L,Vmax值分别为2.70mg/L.min和3.25mg/L.min。The rude laccase from Amanita vema was used to react with 12 commercal dyes.The results showed that 7 dyes could be removed. This paper studied the effects of two dyes cultivating time temperature, pH value and concentration of dye on decoloring capability. The results also showed that the two dyes could react completely with the rode laccase during 1 horns for direct black G and neutroflavine GL. The character absorbance spectra （600nm/440nm） disappeared. The optimal temperature of the rude laccase were both 50℃, and pH value were 5--6 and 3--4. The reaction rate increased with the concentration of the lacease raising but less effect to the totale decolorization rate. The Km of direct black G and neutroflavine GL reacted with the rude laccase were 3.2mg/L and 25. 1mg/L and Vmax were 2.70mg/L·min and 3.25 mg/L·min.

关 键 词：废水处理 染料 脱色 

分 类 号：X788.031[环境科学与工程—环境工程]