表皮生长因子与人绒毛膜促性腺激素共同作用对大鼠卵巢颗粒细胞功能的影响  

Effect of combination of epidermal growth factor and human chorionic gonadotropin on granulosa cells of rat ovary

作　　者：倪江[1,2,3] 刘家慧[1,2,3] 朱辉[1,2,3] 张玮 李秋红[1,2,3] 

机构地区：[1]哈尔滨医科大学生理教研室 [2]中国医学科学院放射医学研究所 [3]黑龙江省第二医院

出　　处：《生殖医学杂志》1998年第1期29-32,共4页Journal of Reproductive Medicine

基　　金：黑龙江省科委资助

摘　　要：为阐明表皮生长因子（ＥＧＦ）对卵巢功能的调节作用，本实验用培养的大鼠卵巢颗粒细胞观察了ＥＧＦ与人绒毛膜促性腺激素（ｈＣＧ）共同作用对细胞增殖、甾体激素与ｃＡＭＰ生成的影响。结果：ＥＧＦ可明显抑制ｈＣＧ刺激的孕酮生成，并呈剂量依赖性，而对ｈＣＧ刺激的雌二醇生成无影响；ＥＧＦ对ｈＣＧ刺激的颗粒细胞ｃＡＭＰ生成具有增强作用，使ｃＡＭＰ生成增加；ＥＧＦ能刺激颗粒细胞增殖，使３ＨＴｄＲ掺入增加，ｈＣＧ不影响ＥＧＦ的这种作用。提示ＥＧＦ与ｈＣＧ对卵巢功能的影响既有相互作用，又有各自的作用。  Objective: To examine the effect of epidermal growth factor (EGF) and human chorionic gonadotropin (hCG) on estradiolc (E2) and progesterone (P4) of granulosa cells of rat ovary in vito.Methods: Immature female wistar rats were treated with DES for 4 days and were sacrificed. Their ovaries were taken out and granulosa cells were collected and cultured, with EGF and hCG, separately or in combination.Roesults: The results showed that hCGinduced production of P4 was inhibited by EGF in a dosedependent manner, but the E2 production induced by hCG was not affected by EGF. EGF enhances cAMP production induced by hCG markedly and increases cAMP content. The proliferation of granulosa cell stimulated by EGF was not influenced by hCG. We could conclude from above data that there were interactions between EGF and hCG, and each has its own role on ovarian function, too.

关 键 词：表皮生长因子 颗粒细胞 CAMP HCG 卵巢 

分 类 号：R339.22[医药卫生—人体生理学]