红豆杉培养物水溶性多糖的提取及清除自由基活性的研究  

Study on Extraction and Scavenging Free Radical Activity of Water Soluble Polysaccharide in Cultivate Taxus

作　　者：刘春兰[1] 杨宇[1] 李转秀[1] 杜宁[1] 

机构地区：[1]中央民族大学生命与环境科学学院,北京100081

出　　处：《中央民族大学学报（自然科学版）》2009年第2期10-15,共6页Journal of Minzu University of China(Natural Sciences Edition)

基　　金：国家"985"课题-中央民族大学"985"重点科研项目(No.cun985-3-3)

摘　　要：红豆杉培养物经热水浸提,乙醇沉淀得粗多糖.粗多糖不含淀粉,凯式定氮法测得蛋白质含量为7.0%.苯酚—硫酸法测定多糖含量为19.71%,硫酸—咔唑法测半乳糖醛酸含量为28.49%.粗多糖经Sevage法和酶法联合脱蛋白,得初步纯化的多糖.在体外设计产生氧离子自由基、羟自由基2个体系,分别加入红豆杉多糖的粗品和精品,结果表明红豆杉多糖对氧离子自由基和羟自由基具有明显的清除效果,且纯化后多糖的清除效果比多糖粗品好.The crude polysaccharide was extracted from cultivate Taxus with hot water, and precipitated by ethanol. The rusults thawed that it was no starch in the crude polysaccharide, The contents of protein was 7.0 % and cultivate Taxus polysaccharide （CTP） was 19.71%. The scavenging capacity for superoxide radical and hydroxyl radical of refined CTP was higher than that of crude CTP.

关 键 词：红豆杉 多糖 提取 自由基 

分 类 号：Q539[生物学—生物化学]