毛细血管扩张性共济失调细胞放射敏感性的研究  

Ataxiatelangiectasia cell sensitivity to ionizing radiation

作　　者：王俊杰[1,2,3] 董秀月[1,2,3] 申文江[1,2,3] 于世平 PiusBrzoska[1,2,3] 李庆琪 

机构地区：[1]北京医科大学第一医院放疗科 [2]中国医学科学院肿瘤医院放射生物室 [3]美国加州大学旧金山分校放疗研究室

出　　处：《中华放射肿瘤学杂志》1998年第1期13-16,共4页Chinese Journal of Radiation Oncology

摘　　要：目的探讨是否毛细血管扩张性共济失调（ＡＴ）细胞互补基因（ＡＴＤＣ）具有抑制ＡＴ细胞放射敏感性的作用和Ｘ线照射后ＡＴ细胞信号传导系统—ＰＫＣδ蛋白表达的改变。材料与方法ＬＭ２１７为ＳＶ４０转化的正常人成纤维基质细胞。ＰＢ０６１为ＳＶ４０转化的ＡＴ病人的成纤维基质细胞。实验分ＬＭ２１７和ＰＢ０６１±四环素（ｌμｇ／ｍｌ）３个组，分别照射不同剂量，求Ｄ０、α、ＳＦ２值。利用抗－ＰＫＣδ和抗ＰＫＣＩ１单抗进行免疫印迹杂交，ＰＢ０６１±四环素（１μｇ／ｍｌ）组分别照射０和５Ｇｙ。结果ＰＢ０６１＋四环素（ＡＴＤＣ－）组的Ｄ０值为０．４５Ｇｙ，α值为２．２１Ｇｙ－１，ＳＦ２为０．０１；而ＢＰ０６１（ＡＴＤＣ＋）组的Ｄ０值为０．５６Ｇｙ，α值为０．９６Ｇｙ－１，ＳＦ２为０．１０。其中α和ＳＦ２值统计学处理Ｐ＜０．０１，提示ＡＴＤＣ基因可以降低ＡＴ细胞的放射敏感性。Ｃａ２＋－非依赖蛋白激酶Ｃδ（ＰＫＣδ）在ＰＢ０６１细胞中表达水平较低，５Ｇｙ照射和四环素处理后均没有改变。ＰＢ０６１细胞中含有蛋白激酶Ｃ的抑制物（ＰＫＣＩ１），５Ｇｙ照射后和四环素处理后其蛋白表达没有改变。结论（１）ＡＴＤＣ基因具有抑?Purpose To investigate whether ATDC gene can lower the radiosensitivity of AT cells and influence the expression of PKCδ protein in AT Cells after ionizing radiation.Materials and Methods LM217 is a normal human fibroblast cell line transformed with SV40. PB061 is an AT Patient's fibroblast cell line transformed with SV40.The values of Do,α and SF2were measured by clone formation after various radiation doses on LM217,PB061± tetracycline(1μg/ml).WesternBlot analysis was performed using anti PKCδ and anti PKCI1 antibody to detect the expression of PKCδ and PKCI1 Protein. Results The values of Do,α and SF2 in PB061+ tetracycline(ATDC-) were 0.45Gy,2.21Gy-1 and 0.01 respectively; and those in PB061(ATDC+) were 0.56Gy,0.96Gy-1 and 0.10 respectively,the difference of α and SF2 value in PB061±tetracycline was significant.The expression of Ca2+independent PKCδ protein in PB061 was much less abundant,and did not show any change after 5Gy irradiation and tetracycline administration.The PKCI1 protein could be induced in PB061 cells and these gave no change in their expressive level after 5Gy irradiation and tetracycline administration.Conclusion (1)ATDC gene can lower the radiosensitivity of AT cells and the α value may be a major factor;(2) The PKCδ dose is not involved in the signal transduction pathway in PB061 cell after ionizing radiation.

关 键 词：毛细血管 扩张性共挤失调 ATDC 放射敏感性 

分 类 号：R818[医药卫生—放射医学]