抑制性受体CD305表达在移植肾排斥反应监测中的意义  

Role of an inhibitory receptor CD305 in renal allograft rejection

作　　者：李州利[1] 蔡明[1] 陈莉萍[1] 张赟[2] 徐竹蔚[2] 金伯泉[2] 石炳毅[1] 

机构地区：[1]解放军总医院第二附属医院器官移植中心,北京100091 [2]第四军医大学免疫教研室

出　　处：《中华泌尿外科杂志》2009年第3期160-162,共3页Chinese Journal of Urology

基　　金：全军医药卫生科研基金资助项目（06G115）

摘　　要：目的探讨抑制性受体CD305的表达在移植肾排斥反应中的意义。方法应用双单克隆抗体夹心ELISA法检测153例肾移植术后患者血清中可溶型CD305（5CD305）的表达水平，20例健康志愿者标本作为对照组。结果对照组和98例移植肾功能正常患者血清sCD305表达分别为（4．3±2．3）和（6．3±3．7）μg／L。20例移植肾急性排斥及5例移植肾失功患者血清sCD305表达明显增加，分别为（36．3±14．7）和（28．8±9．4）μg／L，显著高于对照组和移植物功能正常组（P〈0．01）。30例移植肾慢性排斥及6例尿毒症透析患者血清sCD305分别为（13．1±5．5）和（11．2±4．6）μg／L，亦明显高于对照组和移植肾功能正常组（P=0．00）。结论发生移植肾排斥反应的患者血清sCD305有较高水平的表达，可望作为移植肾排斥反应的监测指标之一。Objective To investigate the role of soluble CD305 （sCD306） in renal allograft rejection. Methods Concentration of serum sCD305 was detected on 20 healthy volunteers and 153 cases of recipients after kidney transplantation by using double monoclonal antibody sandwich enzyme linked immunosorbent assay. Results In the healthy volunteers and 98 recipients with normal renal function, sCD305 was detected at low levels of （4.3±2.3）μg/L and（6.3±3.7）μg/L. In 20 cases of acute rejection and 5 cases of graft loss, serum sCD305 levels were （36. 3 ±14. 7） μg/L and （28. 8±9.4） μg/L, and significantly higher than those in the healthy volunteers and recipients with normal renal function. Meanwhile, in the 30 cases of chronic rejection and 6 cases under dialysis treatment, the levels of sCD305 were （13.1±5.5）μg/L and（11. 2±4.6）μg/L and significantly higher than those in the healthy volunteers and recipients with normal renal function. Conclusion sCD305 was presented at high level in the recipients with renal acute or chronic rejection, and it might be a potential marker for monitoring graft rejection after transplantation.

关 键 词：肾移植 移植物排斥 双单克隆抗体夹心ELISA 可溶型CD305 

分 类 号：R686[医药卫生—骨科学]