阿胶强骨口服液对去卵巢大鼠骨质疏松模型Ⅰ型胶原基因和蛋白表达的影响(英文)  

Effects of donkey-hide glue reinforcing bone oral solution on expression of type Ⅰ collagen gene and protein expression in ovariectomized rats

作　　者：郭向飞[1,2] 倪家骧[2] 沈霖[1] 杨卫红[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院,武汉430022 [2]首都医科大学宣武医院疼痛诊疗科

出　　处：《中国骨质疏松杂志》2009年第5期364-367,共4页Chinese Journal of Osteoporosis

基　　金：湖北省卫生厅资助项目(2008Z-Y18)

摘　　要：目的研究阿胶强骨口服液（donkey-hide glue reinforcing bone oral solution,DGRBOS）对去卵巢骨质疏松模型SD大鼠I型胶原基因和蛋白表达的影响,探讨DGRBOS治疗骨质疏松的疗效机制。方法6月龄SD大鼠36只,随机分为A组（假手术组）,B组（卵巢切除＋生理盐水组）,C组（卵巢切除＋阿胶强骨口服液组）,每组12只。6个月后取材检测。采用荧光定量PCR对I型胶原基因进行定量分析,采用免疫印迹法对I型胶原蛋白进行分析。结果C组I型胶原蛋白与B组比较明显增高,已接近A组（P〈0.01）,差异有明显的统计学意义。I型胶原基因荧光定量PCR（FQ-PCR）结果B组与C组相比,（P〈0.01）,说明扩增效率的差异有明显的统计学意义。结论阿胶强骨口服液可以上调骨组织Ⅰ型胶原mRNA的表达水平,同时显著提高Ⅰ型胶原蛋白表达含量,这是阿胶强骨口服液治疗骨质疏松的机制之一。Objective To investigate the effects of donkey-hide glue reinforcing bone oral solution （DGRBOS） on expression of type Ⅰ collagen gene and protein expression in rats, and explore the mechanism of treating osteoporosis with DGRBOS. Methods 36 female SD rats aged 6 months were randomly divided into 3 groups after ovariectomy： A（ sham group）, B （ group of osteoporotic animal model with normal physiological saline）, C （ treated group of osteoporotic animal model with DGRBOS）. Each group included 12 rats. The expression of type Ⅰ collagen gene in bone tissue was detected by real time fluorescence quantitative PCR（FQ-PCR） method and type collagen protein analysis by Western blotting. Results Type Ⅰ collagen protein of Group C （OVX ＋ DGRBOS） was significandy higher than B group （ovariectomized ＋ saline group）, P = 0.002 （P 〈 0.01）, there are obvious differences in statistical significance. Type Ⅰ collagen gene fluorescence quantitative PCR （FQ-PCR） results of the B group and C group, P = 0.004 （P 〈 0.0l ）, that amplified the differences in the efficiency of a clear statistical significance. Conclusion DGRBOS can increase type Ⅰ collagen mRNA expression level and at the same time significantly increase the type Ⅰ collagen content of expression, which is one of the mechanisms that DGRBOS can be treated for Osteoporosis.

关 键 词：阿胶强骨口服液 Ⅰ型胶原 骨质疏松 

分 类 号：R31[医药卫生—基础医学]