三七总皂甙对骨髓基质细胞成骨分化及成骨作用的调节  

Effects of total saponins of panax notoginseng on osteogenesis of rat bone marrow stromal cells

作　　者：李学东[1] 陈斌[1] 郑创义[1] 刘钊勇[1] 杜世新[1] 

机构地区：[1]汕头大学医学院第一附属医院骨科

出　　处：《中国骨质疏松杂志》2009年第5期371-373,326,共4页Chinese Journal of Osteoporosis

基　　金：广东省中医药科研基金资助项目(2008163);广西梧州制药(集团)股份有限公司科研基金资助项目

摘　　要：目的探讨三七总皂甙对骨髓基质细胞体外成骨分化及成骨作用的影响。方法分离、纯化大鼠骨髓基质细胞（bone marrow strowal cells,BMSCs）,采用常规培养液、成骨诱导液及含100 mg/L PNS的成骨诱导液培养,对各组细胞进行形态学观察,采用MTT法、对硝基苯磷酸盐法、茜素红染色方法及ELISA法观察各组细胞增殖、ALP活性、矿化结节的形成及RANKL的表达。结果骨诱导组、PNS组较培养液组细胞增殖明显（P〈0.05）,而PNS组高于骨诱导组（P〈0.05）;骨诱导组、PNS组ALP活性、钙结节形成高于培养液组（P〈0.01）,PNS组高于骨诱导组（P〈0.05）;骨诱导组、PNS组RANKL表达低于培养液组（P〈0.05）,PNS组低于骨诱导组（P〈0.05）。结论BMSCs成骨诱导过程中,PNS可促进其成骨分化、下调其RANKL表达从而具有抑制破骨细胞生成的潜能。Objective To investigate the effects of total saponins of panax notoginseng on osteogenic differentiation and osteogenesis of rat bone marrow stromal stem ceils（ BMSCs）. Methods BMSCs were isolated from femur and tibia of rats and cultured in basal medium, osteogenic-induced medium and osteogenic-induced medium containing PNS 100 mg/L. The proliferation of BMSCs was evaluated by MTT assay, the osteogenic differerntiation was determined by alkaline phosphatase （ALP） activity and Alizarin Red S staining. The expression of RANKL was detected by enzyme-linked immunosorbent assay. Results During osteogenic differentiation, the proliferation, ALP activity and Calcium deposit were increased and the expression of RANKL were decreased compared with basal medium, respectively （P 〈 0.05 ）. All effects of osteogenic-induced medium on BMSCs were enhanced by PNS. Conclusions The osteogenic differentiation of BMSCs were promoted by PNS and the down-regulation expression of RANKL is the potential mechanism of PNS on osteoclastogenesis.

关 键 词：三七总皂甙 骨髓基质细胞 核因子κB受体激活蛋白配体 成骨作用 

分 类 号：R932[医药卫生—生药学]