In vitro Plant Regeneration of Pepper Cytoplasmic Male Sterility (CMS) Lines via Cotyledon Culture  

辣椒胞质雄性不育系(CMS)子叶培养植株再生(英文)

作　　者：邓明华[1,2] 文锦芬[3] 邹学校[1] 

机构地区：[1]中南大学隆平分院,湖南长沙410125 [2]云南农业大学园林园艺学院,云南昆明650201 [3]昆明理工大学现代农业工程学院,云南昆明650224

出　　处：《Agricultural Science & Technology》2009年第1期39-42,共4页农业科学与技术（英文版）

基　　金：Supported by "863" High Tech Project of China (2001AA241121-10) ;Natural Science Foundation of Yunnan Province (2005C0023Q)~~

摘　　要：An in vitro shoot regeneration procedure was developed in pepper （ Capsicum annuum L. ） cytoplasmic male sterility （CMS） lines 9704A and 8214A using cotyledon as explant. The callus and bud cluster derived from cotyledon tissue explants were proliferated on Murashige and Skoog （MS） medium supplemented with different combinations of 6-benzladenine （6-BA）, indole-3-acetic acid （IAA）, gibberellic acid （GA3） and silver nitrate （AgNO3）. From the formula of MS appended with 5.0 mg/L 6-BA, 1.0 mg/L IAA and 5.0 mg/L AgNO3, for the explants callus and bud cluster, the maximum differentiation rates （ respectively 100.0% and 58.3% ） and average number of adventitious bud from each explant （respectively 18.8 and 13.2） were obtained. The optimum medium combination for the elongation of adventitious bud was determined to be： MS ＋ 3.0 mg/L 6-BA ＋ 1.0 mg/L IAA ＋ 5.0 mg/L AgNO3 ＋ 2.0 mg/L GA3, from which the elongation rates of buds from callus and bud cluster were both 100%, and the average number of per explant adventitious bud number reached 6.3 and 5.8, respectively. And all the elongated shoots were successfully rooted on half-strength MS medium supplemented with 0.3-0.5 mg/L IAA.笔者以细胞分裂素、生长素、赤霉素、硝酸银为培养基的附加成分，利用辣椒CMS9704A和8214A子叶作为外植体，研究辣椒CMS子叶再生体系，为辣椒CMS的遗传改良奠定基础。

关 键 词：Capsicum annuum L. CMS line Cotyledon culture Plant regeneration 

分 类 号：S641.3[农业科学—蔬菜学]