Polymorphism Detection of the Twelfth Exon of Equine MxA Gene  

马MxA基因第12外显子多态性检测(英文)

作　　者：菊林花[1] 金花[2] 呼都特[1] 

机构地区：[1]内蒙古农业大学动物科学与医学学院,内蒙古呼和浩特010018 [2]内蒙古锡林郭勒职业学院医学系,内蒙古锡林浩特市026000

出　　处：《Agricultural Science & Technology》2009年第1期85-88,共4页农业科学与技术（英文版）

基　　金：Supported by the Natural Science Foundation of Inner Mongolia Au-tonomous Region (200508010413)~~

摘　　要：[Objective] The study aimed to establish a fast and accurate method to detect the polymorphism of the 12^th exon of equine MxA gene. [Method] The 12^th exon of MxA gene was amplified by mismatch PCR and the products were analyzed by restriction fragment length polymorphism （RFLP） to determine the point mutation at the 1 790 nt of MxA cDNA. The sequence of the PCR products was also analyzed. [Result] There were three genotypes （AA, AB and BB） in the 12^th exon of equine MxA gene; the 2 081 nt of MxA cDNA mutated from G to C, correspondingly changing the 562^th amino acid of the coding region of MxA protein from tryptophan to cysteine; the specific sequence of the PCR products amplified by mismatch PCR-RFLP was consistent with the analysis results of RFLP. [ Conclusion] The mismatch PCR-RFLP was an easy method with accurate results to detect the polymorphism of the 12^th exon of equine MxA gene.MxA蛋白是Ⅰ型干扰素（IFNα／β）所诱导产生的蛋白之一，属于大分子（70～80kD）GTP酶，能抵抗各种RNA病毒和部分DNA病毒。MxA蛋白结构与其抗病毒活性有直接的关系。有报道表明，MxA蛋白活性部位的一个氨基酸序列的变换，会导致其抗病毒活性的改变。MxA基因多态性分析是研究马MxA蛋白基因抗病毒作用和寻找马MxA蛋白抗性基因的基础。

关 键 词：HORSE MxA gene POLYMORPHISM 

分 类 号：S821[农业科学—畜牧学]