不同水肥条件下番茄土壤微生物群落多样性及其与产量品质的关系  

RELATION OF SOIL MICROBIAL DIVERSITY TO TOMATO YIELD AND QUALITY UNDER DIFFERENT SOIL WATER CONDITIONS AND FERTILIZATIONS

作　　者：韦泽秀[1,4] 梁银丽[1,2] 山田智[3] 曾兴权[4] 周茂娟[1] 黄茂林[1] 吴燕[1] 

机构地区：[1]西北农林科技大学资源环境学院,陕西杨凌712100 [2]中国科学院水利部水土保持研究所,陕西杨凌712100 [3]日本鸟取大学农学部,日本鸟取680-8553 [4]西藏自治区农牧科学院农业研究所,拉萨850002

出　　处：《植物生态学报》2009年第3期580-586,共7页Chinese Journal of Plant Ecology

基　　金：中国科学院西部行动项目(KZCX2-XB2-05-01);中国科学院知识创新重要方向项目(KZCX2-YW-443-3);国家科技支撑项目(2006BAD09B07);中国科学院安塞站和中国科学院水土保持研究所领域前沿项目(SW04302)

摘　　要：采用内含子切接点引物(Intron-splice junction primer)和长随机引物的PCR(Polymerase chain reaction,聚合酶链)标记技术,就黄土高原不同水肥处理对日光温室番茄土壤微生物群落多样性进行了研究,并对产量、品质的影响进行了分析。结果表明,所用的6个引物共能扩增出182条稳定清晰的条带,其中142条为多态性条带,多态性条带为78.02%。聚类分析与主坐标分析表明,水肥对土壤微生物群落多样性的变化有不同的影响。土壤微生物群落多样性指数与番茄产量、果实的Vc、可溶性蛋白质含量成正相关,尤其与Vc的相关系数达0.9211,而与可溶性固形物含量成负相关关系。6个水肥组合中WmFh(中水高肥)土壤微生物群落多样性指数最高,且番茄果实的Vc、可溶性蛋白质含量及产量显著高于其他处理,可溶性固形物含量较低。该组合有利于土壤微生物群落的多样性和稳定性的提高,利于土壤生态环境的改善和番茄优良品质的形成。Aims Our objective was to investigate diversity within soil microbial communities and tomato yields and qualities under different soil water and fertilization treatments. Methods PCR （polymerase chain reaction） with long random primers and intron-splice junction primers were used to study greenhouse soil microbial diversity with tomato cultivated under different treat-ments. Important findings A total of 182 bands were detected with 6 primer combinations, and 142 （78.02%） of the bands were polymorphic. Soil microbial community diversity was affected by the different soil water contents and fertilizations. Diversity was positively correlated with yields, VC contents and solu-ble protein contents, but negatively correlated with soluble solids contents. The highest soil microbial community diversity index was detected under the treatment of medium water level and high fertilization; the VC contents, soluble protein contents and yields of tomato fruits were significantly higher and the soluble solids of the tomato fruits was lower than those in other treatments. This showed that treatment can enhance the soil microbial community diversity and stability and to improve soil ecological environments and tomato quality.

关 键 词：内含子切接点引物 多样性指数 产量 品质 

分 类 号：S641.2[农业科学—蔬菜学]