侧脑室注射orexinA对大鼠麻醉苏醒的影响  

Effect of intracerebroventricular injection of orexinA on recovery of rats receiving anesthesia

作　　者：张旭[1] 王东[1] 叶建宁[2] 贾小兵[2] 杨天德[1] 

机构地区：[1]第三军医大学附属新桥医院麻醉科,重庆400037 [2]第三军医大学附属新桥医院神经内科,重庆400037

出　　处：《中国现代医学杂志》2009年第8期1168-1171,共4页China Journal of Modern Medicine

基　　金：全军"十一五"医学科研基金资助项目(No:06MB237)

摘　　要：目的研究侧脑室注射orexinA对大鼠麻醉苏醒的影响。方法腹腔注射氯胺酮(75mg/kg)和咪达唑仑(5mg/kg)麻醉大鼠后,侧脑室注射不同剂量orexinA,以大鼠翻正反射消失(LRR)持续时间评价麻醉苏醒时间,以共济失调期评价运动功能恢复,4h后进行morris水迷宫实验检测学习记忆功能。结果同对照组相比,高剂量orexinA治疗组(4nmol)麻醉大鼠LRR持续时间和共济失调期明显缩短(P<0.01)。morris水迷宫定向航行实验中,高剂量orexinA治疗组逃逸潜伏期明显缩短(P<0.01),空间探索实验中,目标象限停留时间明显延长(P<0.05)。而低剂量orexinA治疗组(1nmol)差异无显著性。结论侧脑室注射orexinA可缩短麻醉苏醒时间,并促进麻醉后运动功能和学习记忆功能恢复。[Objective] To determine the effect of intracerebroventricular （icy） injection of orexinA on the recovery of rats receiving general anesthesia. [Methods] After intraperitoneal injection of ketamine （75 mg/kg） and midazolam （5 mg/kg）, different doses of orexinA were administered intracerebroventricularly （icy） to rats. The duration of loss-of-fighting reflex （LRR） were used to evaluate the recovery time,the duration of post-LRR ataxia were used to evaluate the motor function. 4 hours after anesthesia, the learning and memory function were studied by Morris Water Maze test. [Results] Compared with the control group, high dose of orexinA （4 nmol） induced significant decrease in the duration of LRR and post-LRR ataxia （P 〈0.01）. In Morris Water Maze test, high dose of orexinA dere＇eased the escape latency （P 〈0.01） and increased the duration that spent in the target quadrant significandy （P 〈 0.05）. However, there was no significant change in these parameter in low dose orexinA group with respect to the control group.[Conclusion] The icv injection of orexinA in rats shortens the recovery from anesthesia and improves motor function and cognitive performance.

关 键 词：orexinA 麻醉 苏醒 学习记忆 

分 类 号：R614.1[医药卫生—麻醉学] R338.64[医药卫生—外科学]