苯并(a)芘对大鼠睾丸胰岛素样因子3基因表达的影响  

Effect of Benzo (a)pyrene on Insl3 Gene Expression in Testis in Puberty Male Rats

作　　者：赵清[1] 舒为群[1] 曹佳[2] 张勇燕[1] 张亮[1] 付文娟[1] 

机构地区：[1]第三军医大学军事预防医学院环境卫生学教研室,重庆400038 [2]第三军医大学军事预防医学院军事毒理学教研室,重庆400038

出　　处：《环境与健康杂志》2009年第5期377-379,共3页Journal of Environment and Health

基　　金：国家自然科学基金重点项目(30630056);国家科技部西部引导项目(2003BA869C);重庆市重大科技专项(CSTC2006AA7003)

摘　　要：目的观察青春期大鼠苯并(a)芘(BaP)亚慢性染毒对睾丸间质细胞功能标志物胰岛素样因子3(Insl3)mRNA表达水平的影响。方法72只青春期健康雄性SD大鼠随机分为溶剂对照组(玉米油)、1、5 mg/kg BaP染毒组,每组24只。隔日染毒,等体积灌胃,连续染毒90 d。分别于染毒后30、60和90 d,每组各随机选取8只大鼠处死。取睾丸、附睾、心、肝、脾、肾称重并计算脏器系数,放免法检测血清睾酮含量,实时荧光定量PCR法检测睾丸Insl3 mRNA表达差异。结果60dBaP各染毒组以及90d5mg/kg组附睾系数显著低于对照组(P<0.05);染毒30d5mg/kg组肝脏系数显著大于对照组(P<0.05),90 d时显著小于对照组(P<0.05);血清睾酮水平30 d和60 d 5 mg/kg染毒组明显高于对照组(P<0.05),90 d 5 mg/kg组明显低于1 mg/kg组(P<0.05);BaP处理组睾丸Insl3 mRNA表达水平各时相点比对照组显著下调(P<0.01),染毒60 d比30 d显著下调(P<0.05),尤其是5 mg/kg组(P<0.01)。结论青春期大鼠BaP亚慢性暴露可以干扰血清睾酮及睾丸Insl3基因表达水平,对附睾、肝脏可能有潜在毒性。Objective To explore the effect of sub-chronic exposure to benzo（a）pyrene （BaP） on insulin-like factor 3 （Ins13）mRNA expression in the testis in puberty male rats. Methods Seventy-two male Sprague-Dawley rats aged 4-5weeks were randomly divided into 3 groups, 24 in each. The animals were exposed to BaP at the doses of 0, 1 and 5 mg/kg respectively by gavage, alt.dieb. for 90 consecutive days. After 30,60 and 90 days of exposure, 8 rats from each group were sacrificed. The body, testis, epididymis and main organ were weighed. Serum testosterone concentration was determined by radioimmunoassay （RIA）. The expression of Ins13 gene in the testis was measured by reahime PCR. Results Compared with the control, in 1 and 5 mg/kg exposure groups the epididymis weight decreased significantly after 60 or 90 days of exposure （P〈0.05）; the liver weight increased in 5 mg/kg group after 30 days of exposure followed by a decrease after 90 days of exposure （P〈0.05）; an increase in serum testosterone was found in BaP treatment group after 60 days of exposure compared with the control （P〈0.05）, while a decline was observed in rats exposed to 5 mg/kg BaP after 90 days of exposure compared with 1 mg/kg group （P〈0.05）. The expressions of Insl3 gene was significantly down regulated in all dosage groups （P〈0.01）, the level of down regulation was more significant after 60 days of exposure than after 30 days of exposure （P〈0.05）,especially in 5 mg/kg group（P〈0.01 ）. Conclusion Subchronic exposure to BaP can induce changes in serum testosterone and Ins13 mRNA expression in the testis, and has potential adverse effect on the epididymis or liver in puberty male rats.

关 键 词：苯并(A)芘 胰岛素样因子3 睾酮 

分 类 号：R994.6[医药卫生—毒理学]