吖啶橙-溴酸钾-萘酚光谱性质及其在痕量尿酚测定中的应用研究  

Spectroscopic Property of AO-H_2SO_4-KBrO_3-Naphthol System and Its Application in Determination of Trace Naphthols in Urine

作　　者：陈云生[1] 杨红梅[1] 王永生[1] 黎俊宏[1] 张锦泉[1] 

机构地区：[1]南华大学公共卫生学院卫生检验教研室,湖南衡阳421001

出　　处：《环境与健康杂志》2009年第5期445-447,共3页Journal of Environment and Health

基　　金：湖南省教育厅科研项目(402JY02C401)

摘　　要：目的研究吖啶橙-溴酸钾-萘酚体系的光谱性质及其共振光散射增强机制,建立共振光散射法测定人尿中痕量萘酚新方法。方法在稀硫酸介质中,萘酚与溴酸钾、吖啶橙(acridine orange,AO)发生反应形成聚集离子,导致共振光散射(resonance light scattering,RLS)显著增强,产生新的RLS光谱。研究了反应体系的吸收、荧光、RLS光谱特征,适宜的反应条件。结果α-萘酚和β-萘酚最大的RLS峰均位于468.0 nm处,线性范围分别为1.41×10-7～2.80×10-5 mol/L,1.28×10-7～3.00×10-5 mol/L,相关系数分别为0.999 6,0.999 3,检出限分别为0.422×10-7 mol/L和0.385×10-7 mol/L。RSD为4.8%～7.3%,平均回收率为90.7%(n=6)。结论该方法灵敏度高,便捷,成本低,可用于检测人尿中的痕量α-萘酚,结果满意。Objective To understand the spectroscopic property of AO-H2SO4-KBrO3-naphthol system and the enhanced mechanism of resonance light scattering （RLS）, and to develop a method for the determination of trace naphthols in urine. Methods In the dilute H2SO4 medium, naphthols could react with KBrO3 and AO to form ion-association complexes, which produced a new RLS spectrum and resulted in the great enhancement of RLS. The characteristics of RLS spectrum, three-dimensions fluorescence spectrum, absorption spectrum and fluorescence spectrum and the optimum conditions of reaction were studied. Results The enhanced intensity of RLS was 468 nm. The linear range was at 1.41×10^-7-2.80×10^-5 mol/L for α-naphthol, 1.28×10^-7-3.00×10^-5 mol/L for β-naphthol. The relative coefficient and the limits were 0.999 6 and 0.422×10^-7 mol/L,0.999 3 and 0.385×10^-7 mol/L for α-naphthol and β-naphthol, respectively. The urine samples analysis of the relative standard deviation was 4.8%-7.3% and the average recovery rate was 90.7%（n=6）. Conclusion This method is sensitive, simple, rapid and applicable to the determination of trace naphthols in human urine.

关 键 词：分光光度法 共振光散射 萘酚 吖啶橙 尿 

分 类 号：O657.3[理学—分析化学]