软骨多糖对荷瘤小鼠体液免疫功能的影响  

Influence of Cartilage Polysaccharide on Humoral Immunity Function of Tumor-Bearing Mice

作　　者：张国蓉[1] 张程[1] 刘安军[1] 车伟[1] 

机构地区：[1]天津科技大学食品工程与生物技术学院,天津300457

出　　处：《现代食品科技》2009年第5期466-468,共3页Modern Food Science and Technology

基　　金：国家自然科学基金资助项目(20776113)

摘　　要：研究软骨多糖对荷瘤小鼠的作用,并探讨其对免疫功能的影响。采用小鼠肉瘤S180细胞建立动物腹水瘤模型,然后随机将小鼠分为空白组,模型组和软骨多糖给药组,连续腹腔注射生理盐水或软骨多糖,用单向琼脂扩散免疫实验测定小鼠抗体,用酶联免疫吸附剂法(ABC-ELISA)测定小鼠外周血细胞因子IL-2、IL-6和TNF-α。在软骨多糖的作用下,荷瘤小鼠分泌的IgG抗体明显增加,IL-2的含量由(160.1±21.24)pg·mL-1降至(120.84±12.05)pg·mL-1,IL-6的含量由(68.32±10.43)pg·mL-1升至(99.34±30.93)pg·mL-1,TNF-α的含量由从(0.08±0.019)pg·mL-1提高到了(0.16±0.006)pg·mL-1,从而增强了小鼠的免疫力。由此得出结论,软骨多糖能通过增强S180荷瘤小鼠的体液免疫功能而抑制肿瘤的生长。Effect of cartilage polysaccharide on the humoral immunity of S180 tumor-bearing mice was studied in this paper. Mouse ascites tumor model was set up using mice sarcoma180 cells and the tested mice were randomly divided into 3 groups （control, model and treated group）. Saline or cartilage polysaccharide was injected into the mice by i.p. method. Humoral immunity was detected by single immunodiffusion test and cytokine （IL-2, IL-6 and TNF-α） of peripheral blood was tested by ABC-ELISA method. Single immunodiffusion test showed that IgG antibody was significantly promoted in tumor-beating mice under the effect of cartilage polysaccharide. The content of the IL-2 decreased from （160.1±21.24）Pg·mL-1 to （120.84±12.05） Pg·mL-1, while the content of IL-6 and TNF-α increased from （68.32±10.43, 0.08±0.019）Pg·mL-1 to （99.34±30.93, 0.16±0.006）Pg·mL-1. It was concluded that the cartilage polysaccharide can improve humoral immunologic function of S180 tumor beating mice and inhibit the tumor growth.

关 键 词：软骨多糖 小鼠肉瘤S180 单向琼脂扩散 ABC-ELISA 

分 类 号：R285.5[医药卫生—中药学]