腺病毒介导人白介素-10转基因对大鼠同种异体移植血管IL-2、TNF-α的影响  

Effect of Adenvirus-mediated Gene Transfer of Human Interleukin-10 on IL-2 and TNF-α of Rat Artery Allograft

作　　者：刘海波[1] 吴伟平[1] 杨顺章[1] 陈宏伟[1] 

机构地区：[1]福建医科大学附属泉州第一医院心外科,福建省泉州市362000

出　　处：《医学理论与实践》2009年第5期499-502,共4页The Journal of Medical Theory and Practice

基　　金：福建省科技厅青年人才项目基金资助(2006F3144)

摘　　要：目的:观察腺病毒介导人白介素-10(hIL-10)转基因对大鼠同种异体移植血管IL-2、TNF-α的影响及其可能的作用机制。方法:以Wistar大鼠为供体,SD为受体,采用颈动脉显微外科原位吻合技术,将经直接浸泡法转染腺病毒介导hIL-10的离体供血管植入受体血管。随机分为3组:I组(空白对照组,n=9),II组(空载体组,n=10),III组(基因转染组,n=10)。移植后5d,观察移植物病理组织学变化及免疫排斥级别;RT-PCR、ELISA、免疫组化染色检测移植血管hIL-10基因产物;免疫组化染色检测IL-2、TNF-α。结果:基因转染组移植血管有效地表达特异性hIL-10基因产物。与对照组比较,急性排斥级别降低(P<0.01);IL-2、TNF-α的表达量明显下降(P<0.01)。结论:腺病毒介导人白介素-10转基因能有效地下调IL-2、TNF-α表达,诱导移植血管局部免疫抑制状态,抑制同种异体移植血管的急性排斥反应。Objeetive：The goal of this study was to investigate the effect of IL-2 and TNF-α of rat allograft arterial transplantation, and discuss the mechanisms. Methods： Orthotopic carotid artery transplantation was performed using Wistar rats as donors and using SD rats as recipients by microsurgical technique. Ex vivo donor was transfected Ad. hIL-10 for 30 minutes by snaking method. SD rats were divided into three groups：group Ⅰ（n=9） ,receiving natural solution; group Ⅱ（n= 10）, receiving empty adenovec, group Ⅲ（n= 10）, receiving adenovec-hIL-10 complex. 5 days after transplantation, the grafts were harvested; transgene expression of hIL-10 was detected by means of both RT-PCR, ELISA and immunohistochemistry; the repression of IL-2, TNF-α in artery allograhs were detected by using immunohistochemial staining method; histologic rejection score; pathological morpbologic change was analyzed. Results： 5 days after transplantation in group Ⅲ, transgene expression of human interleukin-10 was detected by means of both ELISA, RT-PCR and immunohistochemistry. The rejection score in group Ⅲ was significantly lower than that in group Ⅰ , Ⅱ. The expression of IL-2,TNF-α （P〈 0. 01）. Conclusion： The expression of IL-2, TNF-α were successfully reduced bY Ad. hIL-10; Ad. hIL-10 induced graft local immunical inhibiting surcuation. Ad. hlL-10 gene transfer ex vivo into rat artery allografts can inhibit acute rejection reaction of allograft arterial transplantation.

关 键 词：人白介素-10 转基因治疗 IL-2 TNF-Α 同种异体血管 大鼠 

分 类 号：R654.3[医药卫生—外科学]