阿霉素中毒大鼠肺组织中β-防御素-2的基因表达  

Expression of β-defensin-2 gene in the lung tissue of rats intoxicated by adriamycin

作　　者：于影[1] 胡杰[1] 关宿东[1] 

机构地区：[1]蚌埠医学院生理学教研室,安徽蚌埠233030

出　　处：《蚌埠医学院学报》2009年第5期376-378,共3页Journal of Bengbu Medical College

基　　金：安徽省高校省级自然科学研究资助项目(kj2007B018)

摘　　要：目的:探讨阿霉素(adriamycin,ADM)所致大鼠急性肺损伤对肺组织β-防御素-2(BD-2)基因表达的影响。方法:雄性SD大鼠40只,随机分成ADM低剂量组(10 mg/kg)、中剂量组(20 mg/kg)、高剂量组(40 mg/kg)及对照组,每组10只。模型组分别一次性腹腔注射不同剂量ADM制备急性肺损伤模型;对照组用生理盐水腹腔注射。3天后剖胸取肺,采用半定量RT-PCR技术检测肺组织BD-2 mRNA表达的变化。结果:ADM中、高剂量组大鼠BD-2基因表达均较对照组明显上调(P<0.01),低剂量组与对照组无统计学意义(P>0.05)。结论:ADM所致大鼠急性肺损伤中,肺组织BD-2的mRNA表达水平上调。Objective：To observe the influence of acute pulmonary injury induced by adriamyein（ADM） on β-defensin-2 （BD-2） gene expression in rats. Methods： Forty male Sprague-Dawley rats were randomly divided into four groups： low close ADM group, medium dose group,high close group and control group. The ADM groups were administered ADM of different doses （ 10,20 and 40 mg·kg^-1·d^-1）. Meanwhile, normal saline was given to the control group. All the rats were killed three days later, and the lung tissues were removed by thnraeotomv. The expression of BD-2 at mRNA level was determined by RT-PCR analysis. Results：The level of BD-2 geue expression in medium-dose and high-dose groups was up-regulated significantly compared with that in the control group （P 〈0. 01 ）; and the difference had no statistical significance between the low-dose group and the eontrnl （P 〉0.05 ）. Conclusions：The level nf BD-2 gene is up-regulated in the lung tissues of rats with acute puhnnnary injury induced by ADM.

关 键 词：阿霉素 肺 Β-防御素-2 基因表达 大鼠 

分 类 号：R979.14[医药卫生—药品]