毛细管电泳氨类手性药物对映体拆分机理研究  

Study of the enantiomers resolution mechanism of chiral amide pharmaceuticals by capillary electrophoresis

作　　者：金瑛芝[1] 王园朝[1] 成美容[1] 盛春荠[1] 

机构地区：[1]杭州师范学院材料与化学化工学院,有机硅化学与材料技术教育部重点实验室,浙江杭州310036

出　　处：《化学研究与应用》2009年第5期711-714,共4页Chemical Research and Application

基　　金：浙江省自然科学基金项目(Y406237)资助;浙江科技厅大型仪器测试基金项目(2007F70051)资助

摘　　要：Using capillary zone electrophoresis,the enantiomers resolution of 13 pharmaceuticals including non-amide,primary amine,the secondary amine and the tertiary amine was studied with highly sulfated – α(β,γ)-cyclodextrins(HS-α(β,γ)-CD) as chiral selector,It was found a good resolution effect for the amide chiral pharmaceuticals,especially for tertiary amine.Through the primary analysis of chiral resolution mechanism,it was believed there be the static gravitational action in addition to space matches and hydrogen bonding between highly sulfated cyclodextrins and the amine drugs.With mobility shift method,Determination of binding constants between chlorpheniramine,hydrochloride isoprenaline,naphthylethylamine and HS-β-CD showed that the binding constant order of chlorpheniramine > hydrochloride isoprenaline > naphthylethylamine,and that highly sulfonated cyclodextrins and the tertiary amine the strongest action force.Using capillary zone electrophoresis, the enantiomers resolution of 13 pharmaceuticals including non-amide, primary amine, the secondary amine and the tertiary amine was studied with highly sulfated - α（ β,γ）- cyclodextrins （HS-α（ β,γ）- CD） as chiral selector, It was found a good resolution effect for the amide chiral pharmaceuticals, especially for tertiary amine . Through the primary analysis of chiral resolution mechanism, it was believed there be the static gravitational action in addition to space matches and hydrogen bonding between highly sulfated cycledextrins and the amine drugs. With mobility shift method, Determination of binding constants between chlorpheniramine, hydrochloride isoprenaline, naphthylethylamine and HS-13-CD showed that the binding constant order of chlorpheniramine 〉 hydrochloride isoprenaline 〉 naphthylethylamine, and that highly sulfonated cyclodextrins and the tertiary amine the strongest action force.

关 键 词：毛细管电泳 氨类手性药物 对映体拆分 高磺化环糊精 分离机理 

分 类 号：O657.8[理学—分析化学]