高效液相色谱法测定广西产美味猕猴桃根中熊果酸的含量  

Determination of Ursolic Acid in Roots of Acitinidia deliciosa in Guangxi Province by HPLC

作　　者：梁洁[1] 甄汉深[1] 李生茂[1] 马雯芳[1] 刘振杰[1] 张婷[1] 

机构地区：[1]广西中医学院.药学院,广西南宁530001

出　　处：《时珍国医国药》2009年第5期1025-1027,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金地区联合资助项目(No.30160095);广西教育厅科研项目(No.200810LX107);广西高校人才小高地建设创新团队资助计划;广西中医学院高层次人才科研启动基金课题(No.G2006047)

摘　　要：目的测定广西产美味猕猴桃根中熊果酸的含量。方法以熊果酸含量为考查指标,采用正交实验优选提取工艺。采用高效液相色谱法,色谱柱为Thermo Hypersil BDS C18色谱柱（5μm,4.6 mm×250 mm）;柱温20℃;流动相为乙腈-0.1%磷酸水溶液（85∶15）;流速1.0 ml/min;检测波长210 nm。结果熊果酸的浓度在0.012 5～0.150 0 mg/ml时与峰面积呈良好的线性关系,样品的平均回收率为99.35%,RSD为1.52%（n=6）。结论该方法准确可靠,重复性好,适用于美味猕猴桃根药材的质量控制。Objective To determine the ursolic acid in the roots of Actinidia deliciosa in Guangxi province by HPLC. Methods - With the content of ursolic acid as index, orthogonal experiments were used to optimize extraction technology. HPLC method was developed. The separation was performed on Hypersil BDS C18 column （5 μm, 4.6 mm×250 mm）. The column temperature was 20℃. The mobile phase consisted of acetonitrile -0.1% phosphoric acid （85： 15）. The flow rate was 1.0 ml/min. The detection wavelength was 210 nm. Results The result showed that there was good linear relationship between the area and concentration of ursolic acid within the range of 0. 012 5 - 0. 150 0 mg/ml. The average recovery was 99.35% and RSD was 1.52% （n = 6）. Conclusion The method is convenient, sensitive and reliable. It can be used for quality control of Actinidia deliciosa.

关 键 词：高效液相色谱 广西产美味猕猴桃根 熊果酸 

分 类 号：R284.2[医药卫生—中药学]