虫草多糖对8-MOP/UVA诱导皮肤成纤维细胞光老化的拮抗作用  

Antagonism of Cordyceps Polysaccharides to Photoaging Fibroblasts Induced by 8-MOP/UVA

作　　者：李华[1] 叶(目亭)杰[1] 李伯勤[1] 应馨萍[1] 

机构地区：[1]上海中医药大学,上海201203

出　　处：《时珍国医国药》2009年第5期1074-1076,共3页Lishizhen Medicine and Materia Medica Research

基　　金：上海市教育委员委青年基金(No.05cz02);上海市教育委员会重点学科建设项目(No.J50301)

摘　　要：目的探讨虫草多糖(Cordyceps Polysaccharides,CP)对皮肤成纤维细胞光老化的保护作用。方法用8-甲氧补骨脂素联合UVA(8-MOP/UVA)诱导皮肤成纤维细胞光老化,用HE染色、MTT、羟脯氨酸含量、丙二醛含量检测及SOD活力分析等方法观察虫草多糖对成纤维细胞光老化过程中的形态、活力、胶原合成及抗氧化能力的影响。结果光老化组细胞发生形态改变,增殖能力明显下降,羟脯氨酸含量下降,丙二醛含量升高,SOD活力下降,虫草保护组细胞有所改善。结论虫草多糖对8-MOP/UVA诱导皮肤成纤维细胞光老化有拮抗作用。Objective To observe the protective action of cordyceps polysaccharides （CP） on photoaging skin fibroblasts. Methods Photoaging skin fibroblast models were induced by 8 - MOP/UVA. Cordyceps polysaccharides was administrated before 8 - MOP/UVA . HE stained,MTT, hydroxyproline（HYP） , MDA and SOD quantitant were used to test the effects of cordyceps polysaccharides. Results Cell crimple, condensation of nuclear chromatin in 8 - MOP/UVA model group were observed. Compared with model group, cp groups alleviate cell crimple could increase the activity of cell, decrease the content of MDA, and increase the level of HYP and the activity of SOD. Conclusion Cordyceps polysaccharides has protective effect on photoaging skin fibroblast.

关 键 词：虫草多糖(CP) 成纤维细胞 光老化 

分 类 号：R284.2[医药卫生—中药学]