超高效液相色谱法测定龙胆药材中的獐芽菜苦苷和龙胆苦苷  

Determination of Swertiamarin and Gentiopicroside in Root of Gentiana scabra Bge.by UPLC

作　　者：曹悦[1,2] 左代英[2] 吴晓兰[1] 刘敬武 孙启时[2] 

机构地区：[1]辽宁省食品药品检验所,辽宁沈阳110023 [2]沈阳药科大学,辽宁沈阳110016 [3]辽宁天瑞绿色产业科技开发有限公司,辽宁清原113300

出　　处：《时珍国医国药》2009年第5期1079-1080,共2页Lishizhen Medicine and Materia Medica Research

基　　金：国家科技支持计划(No.2006DAI06A05-08)

摘　　要：目的建立超高效液相色谱（UPLC）法测定龙胆药材中活性成分的方法。方法采用L9（34）正交表安排实验,确定最佳提取条件。色谱柱：Shimadzu C18（3.0 mm×75 mm,1.7μm）;流动相：乙腈-0.1%磷酸（V∶V=5.5∶94.5）,流速0.80 ml/min;检测波长235,270 nm;柱温为40℃。结果獐芽菜苦苷、龙胆苦苷分别在0.154 8～1.858 0 mg/ml,0.322～5.152mg/ml范围内呈良好的线性关系,平均回收率分别为102.09%,RSD=1.68%（n=6）;103.80%,RSD=1.11%（n=6）。结论采用UPLC法控制龙胆质量,操作简单,结果准确,方法可行。Objective To establish a method for the determination of swertiamarin and gentiopicroside in root of Gentiana scabra Bge. by UPLC. Methods By L9 （ 34 ） orthogonal design, an optimal condition was confirmed. The separation was performed on Shimadzu C18（3.0 mm ×75 mm,1.7μm） column, the mobile phase was Acetonitrile -0. 1% Phosphorylation（V： V = 5.5： 94.5 ）. The flow rate was 0.8 ml/min, the detection ＇wavelength was 235nm, 270nm, and the column temperature was 40℃. Resuits This method had good linearity in the range of 0. 322 -5. 152mg/ml,and the average recovery was102.09 % with RSD of 1.68% for swertiamarin. It had good linearity in the range of 0. 1548 - 1. 858 mg/ml , and the average recovery was 103.80% with RSD of 1.11% for gentiopicroside. Conclusion This method is simple , accurate , and suitable for the quality control of root of Gentiana scabra Bge. by UPLC.

关 键 词：龙胆 超高效液相色谱 獐芽菜苦苷 龙胆苦苷 

分 类 号：R284.2[医药卫生—中药学]