重庆道地药材青蒿高效液相色谱指纹图谱研究  

Studies on HPLC Fingerprint of Artemisia annua L.from Chongqing

作　　者：胥秀英[1] 郑一敏[1] 傅善权[1] 韩玉梅[1] 杨艳红[1] 

机构地区：[1]重庆工学院.生物工程学院,重庆400050

出　　处：《时珍国医国药》2009年第5期1188-1189,共2页Lishizhen Medicine and Materia Medica Research

基　　金：重庆市教委资助项目(No.KJ060604)

摘　　要：目的建立重庆道地药材青蒿高效液相色谱(HPLC)指纹图谱研究方法。方法采用反相高效液相色谱法,测定青蒿药材指纹图谱,色谱条件为:色谱柱:Shimadzu C18(150 mm×4.6 mm,5μm);流动相:甲醇-水梯度洗脱;流速:1.0 ml.min-1;检测波长:206 nm;柱温:25℃。结果建立了重庆道地药材青蒿指纹图谱标准。结论该指纹图谱方法简便、快速、重现性好,可作为青蒿药材的一项质控指标。Objective To establish an HPLC fingerprint analysis method for identification of Artemisia annua L. from Chongqing. Methods The fingerprint was determined by HPLC. The analysis was carried out on Shimadzu C18 scolumn（ 150 mm ×4.6 mm,5 pore）. The mobile phase was CH3 OH - H2 O（ gradient elution）with a flowrate of 1.0 ml · min^-1 at 206 nm and the seperation was performed at 25℃. Results The standard of fingerprint in Artemisia annua L was established. Conclusion The method is simple, fast and repeatable. It can be used in quality assessment of Artemisia annua L.

关 键 词：青蒿 指纹图谱 模糊模式识别 高效液相色谱 

分 类 号：R284.1[医药卫生—中药学]