低氧训练对大鼠EPO／EPOR及其表达的影响  

Effect of hypoxic training on EPO/EPOR expression in rats

作　　者：赵鹏[1] 路瑛丽[1] 冯连世[1] 徐建方[1] 朱珂 

机构地区：[1]国家体育总局体育科学研究所,北京100061 [2]河南省体育科学研究所,郑州450003

出　　处：《山东大学学报（医学版）》2009年第5期5-15,共11页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金资助项目(30570892)

摘　　要：目的研究低氧训练及复氧训练对大鼠EPO-EPOR的影响。方法经过适应性训练和力竭实验筛选出的60只SD雄性大鼠,分成6组,保证每组大鼠体重,力竭时间、力竭血乳酸等基本一致,双盲抽签确定分组:①低住低练组,②高住高练组,③高住低练组,④低住高练组,⑤高住高练复氧训练组,⑥高住低练复氧训练组。采用水平跑台进行耐力训练,训练强度为常氧下35 m/min,低氧下30 m/min,1 h/d,5 d/周,低氧训练6周,复氧训练1周。最后一次训练后恢复48 h取血和腓肠肌。ELISA法测血清EPO,免疫组化法测定肾脏EPO、骨骼肌EPO、EPOR,实时定量PCR测腓肠肌EPO mRNA和EPOR mRNA表达。结果高住高练组较低住低练组大鼠骨骼肌EPO mRNA和EPOR mRNA表达均有高度显著性升高(P<0.01),高住低练组与低住低练组相比骨骼肌EPO mRNA和EPOR mRNA表达有高度显著性(P<0.01)和显著性(P<0.05)升高。高住高练后复氧训练1周,血清EPO水平有显著性升高(P<0.05),骨骼肌EPO mRNA和EPOR mRNA表达有高度显著性降低(P<0.01),高住低练后复氧训练1周,骨骼肌EPO mRNA表达显著性降低(P<0.05),其余变化不大。EPO主要在肾脏皮质的胞浆中弥漫表达,与常氧安静对照组相比,低住低练组、高住高练组和高住低练组EPO表达较强;与高住高练和高住低练相比,高住高练后复氧训练组和高住低练后复氧训练组EPO表达都略有增强,都比常氧安静组强。结论引起EPO水平变化的原因不是缺氧,而是氧浓度的变化,并且可能会有低氧持续暴露时间和氧浓度适当匹配性。骨骼肌EPO-EPOR mRNA系统的表达受血清EPO浓度的影响,当血清EPO浓度较高时,抑制了骨骼肌EPO-EPOR mRNA系统的表达,骨骼肌EPOmRNA表达与血清EPO有显著负关(r=-0.676),同时,骨骼肌EPO mRNA与EPOR mRNA之间有非常显著相关关系(r=0.918)。Objective To explore the effects of different hypoxia training methods on EPO-EPOR to provide a theoretical and application basis for using hypoxia training. Methods After adaptability training and exhaustive tests, 90 rats were selected from 120 male SD rats （6-week old） and randomly divided into 9 groups： the normoxia quiet control group, the continuing hypoxia group, the intermittent hypoxia group, the normaxia training group, the living-high and training-high group（HiHi）, the living- high and training-low group（HiLo）, the living-low and training-high group（LoHi）, the normoxia training group after HiHi, and the normoxia training group after HiLo. During a 6-week experimental period, 13.6% concentration of oxygen （equal to an altitude of 3 500 m） was introduced into the hypoxic chamber. Rats that had undergone hypoxia training were introduced to a tread- mill. The speed of training was 30 m/min in hypoxia and 35 m/min in normoxia. Then the rats were trained 5 d/week and were sacrificed after 6 weeks. RQ-PCR, immunohistochemistry and Western blot were used to determine the mRNA and protein levels of HIF-lain the skeletal muscle to probe further into the meehanisms of acclimatization in the skeletal muscle. Results Compared with the LoLo group, the mRNA level of EPO and EPOR in beth the HiHi group （ P 〈 0.01 ） and the HiLo group（ P 〈 0.05）significantly increased. In the normoxia training group after HiHi, the mRNA level of EPO in the serum significantly increased （P 〈 0.05）, and the mRNA level of EPO and EPOR in skeletal muscle decreased significantly; while in the normoxia training group after HiLo, the mRNA level of EPO in the skeletal muscle significantly decreased （ P 〈 0.05）. Conclusion It is concentration of oxygen rather than hypoxia that causes changes in the level of EPO, which may be related to exposure time of hypoxia. The mRNA level of EPO in the skeletal muscle is negatively related to that of EPO in the serum（ r = - 0.676）, also, the mRNA levels of E

关 键 词：低氧训练 促红细胞生成素 促红细胞生成素受体 骨骼肌 大鼠 SPRAGUE-DAWLEY 

分 类 号：G804.7[文化科学—运动人体科学]