葡萄糖和胰岛素对脂肪细胞内脂素表达的作用  

Effect of glucose and insulin on visfatin expression in 3T3-L1 adipocytes

作　　者：王坤[1] 王文汇[2] 陈淼[1] 陈福莲[1] 娄宁[2] 

机构地区：[1]山东大学医学院,济南250012 [2]山东大学附属济南市中心医院内分泌科,济南250013

出　　处：《山东大学学报（医学版）》2009年第5期75-77,82,共4页Journal of Shandong University:Health Sciences

摘　　要：目的观察脂肪细胞经不同浓度葡萄糖和胰岛素干预后内脂素表达的改变,探讨内脂素在糖尿病发病过程中的作用。方法培养3T3-L1前脂肪细胞,并诱导分化成成熟脂肪细胞。采用不同浓度葡萄糖、胰岛素孵育脂肪细胞48 h,RT-PCR和western blot技术检测3T3-L1脂肪细胞中内脂素mRNA和蛋白的表达水平。结果与对照组比较,随着葡萄糖浓度的增加,3T3-L1脂肪细胞内脂素mRNA和蛋白的表达量下降(P均<0.05);在不同浓度胰岛素作用下,3T3-L1脂肪细胞内脂素mRNA和蛋白的表达量改变无统计学意义(P>0.05)。结论高糖状态可抑制体外培养的脂肪细胞内脂素的表达,胰岛素浓度的改变对内脂素表达无影响。Objective To investigate the effects of different concentrations of glucose and insulin on expressions of visfatin mRNA and protein in 3T3-L1 adipocytes and to explore the role of visfatin on diabetes mellitns. Methods 3T3-L1 pre-adipocytes were cultured in vitro and differentiated. Mature adipocytes were incubated with different concentrations of glucose and insulin for 48 hours. Then total cellular RNA and protein were extracted, and expressions of visfatin mRNA and protein were determined by semi-quantitative RT-PCR and western blot with β-actin as an internal control. Results Compared with the control group, expressions of visfatin mRNA and protein in mature adipocytes were decreased by high glucose（ P 〈 0.05）, but were not significantly changed by different concentrations of insulin（ P 〉 0.05）. Conclusion In in vitro studies, a high level of glucose can down- regulate visfatin expression, but concentration of insulin has no effect on visfatin expression.

关 键 词：葡萄糖 胰岛素 脂细胞 内脂素 

分 类 号：R587.1[医药卫生—内分泌]