汉坦病毒GM04-38株包膜糖蛋白上融合肽的初步确定  

Defining fusion peptide on glycoprotein of the Hantavirus GM04-38

作　　者：曹海霞[1] 陶泽新[2] 郑晓民[1] 刘晓丽[1] 王桂亭[1] 许洪芝[1] 温红玲[1] 宋艳艳[1] 赵丽[1] 姚苹[1] 王志玉[1,3] 

机构地区：[1]山东大学公共卫生学院病毒学研究室,济南250012 [2]山东省疾病预防控制中心免疫预防管理所,济南250014 [3]实验畸形学教育部重点实验室,济南250012

出　　处：《山东大学学报（医学版）》2009年第5期125-130,共6页Journal of Shandong University:Health Sciences

基　　金：教育部博士点基金项目资助课题(20060422015);山东大学创新团队项目资助

摘　　要：目的初步确定汉坦病毒GM04-38株包膜糖蛋白上潜在的融合肽区域。方法采用基因定点突变技术将潜在融合肽区域的十个关键氨基酸突变成性质相左的氨基酸,转染Vero E6细胞后,间接免疫荧光(IFA)检测糖蛋白表达,采用Giemsa染色法观察细胞融合现象。结果在Vero E6细胞中成功表达出糖蛋白,IFA显示关键氨基酸突变前后细胞中均有荧光信号,呈胞浆分布,但细胞融合现象在突变后消失。结论潜在融合肽区域的十个氨基酸突变均对细胞融合产生明显的影响,提示该段区域很可能是病毒的融合肽。Objective To determine the potential putative fusion region on glycoprotein of the Hantavirus GM04-38. Methods Site-directed mutagenesis was used to construct 10 GP gene mutants, which were then expressed in Vero E6 cells. IFA was performed to determine expression of GPs genes, and Giemsa staining was used to observe cell fusion activities. Results IFA showed that the GPs genes were successfully expressed, and the fluorescent signal was robust and concentrated in the perinuclear region of the transfected cells. But cell fusion activities were not found after mutation. Conclusion Mutation of 10 amino acids can obviously influence cell fusion activities, suggesting that it is possible that fusion peptide is in this region.

关 键 词：汉坦病毒 包膜糖蛋白 细胞融合 融合肽 

分 类 号：R373.32[医药卫生—病原生物学]