2,2′,4,4′-四溴联苯醚对鲫鱼离体肝脏组织的氧化胁迫  

Oxidative Stress of Tetrabromodiphenyl Ether to the Liver of Carassius auratus. in Vitro

作　　者：吴伟[1,2] 聂凤琴[2] 瞿建宏[1] 杨光[2] 

机构地区：[1]中国水产科学研究院淡水渔业研究中心,中国水产科学研究院内陆渔业生态环境与资源重点开放实验室,江苏无锡214081 [2]南京农业大学渔业学院,江苏无锡214081

出　　处：《农业环境科学学报》2009年第5期1005-1009,共5页Journal of Agro-Environment Science

基　　金：中央级公益性科研院所基本科研业务费专项资金项目(2007JBFB02);中国水产科学研究院内陆渔业生态环境与资源重点开放实验室开放课题(YM2007-10)

摘　　要：以鲫鱼（Carassius auratus）为试验材料,通过鲫鱼离体肝脏组织的染毒试验,研究了在离体条件下经不同浓度的2,2′,4,4′-四溴联苯醚（PBDE-47）暴露后,鲫鱼肝脏组织中总抗氧化能力（T-AOC）、丙二醛（MDA）含量、黄嘌呤氧化酶（XOD）和超氧化歧化酶（SOD）活性的动态变化。结果表明,采用浓度为0.10-10.00mg·L^-1的PBDE-47处理鲫鱼肝脏组织30min,0.10mg·L^-1浓度组鲫鱼肝脏组织中T-AOC、MDA含量、XOD和SOD活性与对照组相比无显著差异（P〉0.05）,0.56mg·L^-1以上各浓度组的XOD活性和MDA含量随PBDE-47浓度增加逐渐上升,而T-AOC和SOD活性逐渐下降,均与PBDE-47浓度呈明显的相关关系（P〈0.01）。这说明PBDE-47对鲫鱼肝脏产生了氧化损伤,具有生化毒性影响。The present paper intends to introduce the authors＇ experimental research on the toxic effects of tetrabromodiphenyl ether（PBDE- 47 ） on the content of maleie dialdehyde（MDA）, the total anti-oxidative capacity（T-AOC ） and the activities of xanthine oxidase （XOD） and superoxide dismutase （SOD） in the liver of Carassius auratus exposed to PBDE-47. For the research purpose, we isolated the liver of the fishes to be tested and put them back to water with the concentration of PBDE-47 being, 0, 0.10, 0.56, 1.00, 1.80, 5.60 and 10.00 mg ·L^-1. The four indicators were then analyzed after a 30 min exposure in vitro at the temperature of 25 ℃. Just as the normal values of XOD and SOD would do（ 56.00-1.51 ）U· g^-1 and（ 84.72-0.58 ）U· mg^-l, the results of our experiments proved that the normal values of MDA and T-AOC tended to be separately（ 10.56-0.21 ）nmol· mg^-1 and（9.22~0.12）U·mg^-1. And the test group of 0.10 mg· L^-1 PBDE-47 had no effects on T- AOC ,MDA ,XOD and SOD in the liver after treated 30 min（P〉0.05 ）. But the other test groups, especial above 0.56 mg· L^-1, could cause the obvious changes to T-AOC, MDA, XOD and SOD （P〈0.01）. With the concentration of PBDE--47 ineresed, MDA, XOD tend to increase while T- AOC, SOD were lowering, which turned to prove T-AOC, XOD, MDA, SOD and PBDE-47 concentrations were closely related. The above results can clearly indicate that the oxidative stress and the biochemical toxicity to the liver of Carassius auratus with PBDE-47 is above 0.56 mg· L-1.

关 键 词：2 2′ 4 4′-四溴联苯醚 鲫鱼 肝脏 离体 氧化胁迫 

分 类 号：X503.225[环境科学与工程—环境工程]