粪鬼伞多糖的提纯与定性研究  

Purification of polysaccharides from Coprinus sterqulinus Fr.

作　　者：刘洪霞[1] 田文莹[1] 王志兵[1] 邱芳萍[1] 

机构地区：[1]长春工业大学化学与生命科学学院,吉林长春130012

出　　处：《长春工业大学学报》2009年第2期126-129,共4页Journal of Changchun University of Technology

基　　金：吉林省教育厅科技计划项目(200660)

摘　　要：采用水提醇沉法提取粪鬼伞粗多糖(CPS),并对其提纯与鉴定。CPS经酶法结合Sevag法脱除蛋白,上DEAE-cellulose-52柱和Sepharose-6B凝胶柱层析,分离纯化得多糖CPS-Ⅰa。经紫外光谱扫描,Sephadex G-100凝胶柱层析和红外光谱分析,结果显示,多糖CPS-Ⅰa具有葡萄糖醛酸的特征吸收峰,α-D吡喃糖苷键连接,是一种酸性多糖。With water-ethyl alcohol extraction method, crude Coprinus sterqulinus Fr. Polysaccharide （CPS） is obtained and then purified. CPS is united with Sevag to remove the proteins, upper DEAE-52 column and Sepharose-6B gel to get the component CPS-Ⅰ a. Sephadex G-100 is scanned with UV spectra and then analyzed with IR spectra, and the results indicate that CPS- Ⅰ a, as an acid polysaccharide, has an absorption peak for glucuronidase and α-D glycosidic bond.

关 键 词：粪鬼伞多糖 纯化 定性 

分 类 号：Q539.7[生物学—生物化学]