低致病性禽流感H9N2病毒感染鸡群引起巴氏杆菌的内源性感染  

Low Pathogenic Avian Influenza Virus(LPAIV) H9N2 Inoculation into Chickens Result in Secondary Infection of Pasteurella multocida

作　　者：孙瑞芹[1] 何宏轩[2] 高云英[1] 

机构地区：[1]西北农林科技大学动物医学学院,陕西杨凌712100 [2]中国科学院动物研究所野生动物疫病研究中心,北京100101

出　　处：《中国农学通报》2009年第10期15-18,共4页Chinese Agricultural Science Bulletin

基　　金：中国科学院知识创新工程重要方向项目(KSCX2-YW-N-063);中国科学院动物研究所前沿领域创新项目(KSCX3-IOZ-0617);十一五国家科技支撑计划(2006BAD06A01)

摘　　要：为研究低致病性禽流感H9N2病毒感染鸡群的变化,用107.4EID50/0.1mlH9N2病毒,0.5ml/只,接种1月龄SPF鸡。结果5日后引起鸡群发病,并引起一些个体发生死亡,剖检发现喉头充血、肺淤血,气囊膜增厚,皮下胶样浸润、出血,腺胃乳头出血,十二指肠出血。肝、脾、肾、肺常见有灰黄色坏死灶。无菌取肺组织做细菌培养时发现在血平板上有灰白色露珠状菌落,挑取菌落做碱性美蓝染色镜检发现两极浓染的球杆状菌;经生化和PCR检验确认该菌属于多杀性巴氏杆菌。低致病性禽流感H9N2病毒感染鸡群可引起巴氏杆菌的内源性感染,从而导致鸡群的高发病率与高死亡率现象。To observe the changes of chickens when post-challenge with LPAIV H9N2. One-month-old SPF chickens were inoculated with 1074EIDso/0.1 ml H9N2 viruses, 0.5ml/chick. Results Chickens were sick and a few of them were died after 5days inoculation. At necropsy, the primary gross lesions in all chickens were severe pulmonary consolidation and muhifocal hemorrhage in several organs, including subcutaneous, lung, heart, thymus, stomach, intestine, liver, and lymph nodes; Necrotic foci were in liver, spleen, kidney and lung. Bacterial test in lungs were performed in blood agar plate as previously described and colony showed dewdrop appearance, the colony treated with methylene blue was observed under microscope, which was two-pale deep color. The bacterial isolated was identified as Pasteurella multocida based on biochemical characteristics and PCR. Low pathogenic avian influenza H9N2 virus may cause secondary infection of Pasteurella multocida, which could results of high rate both morbidity and mortality in chickens.

关 键 词：低致病性禽流感 巴氏杆菌 内源性感染 

分 类 号：S858.31[农业科学—临床兽医学]