SSR标记分析小麦品种Flinor和铭贤169的遗传差异  

The Genetic Difference of Flinor and Mingxian169 Analyzed by Microsatellite Marker

作　　者：左丽玲[1,2] 冯晶[2] 蔺瑞明[2] 章振羽[2] 曹远银[1] 徐世昌[2] 

机构地区：[1]沈阳农业大学植物保护学院,沈阳110161 [2]中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京100193

出　　处：《中国农学通报》2009年第10期59-62,共4页Chinese Agricultural Science Bulletin

基　　金：国家"863"计划项目"小麦条锈病和白粉病抗病基因实用分子标记建立及其选择效率评价"(2006AA10Z1C7);国家"973"计划项目"农业生物多样性控制病害的方法及遗传学原理和流行学机制"(2006CB100203);国家自然科学基金项目"小麦抗条锈病近等基因系的创建及应用"和"小麦抗条锈病温敏微效基因发掘;定位及利用研究"(30471131和30671343)

摘　　要：采用微卫星(SSR)分子技术,对小麦品种Flinor与铭贤169染色体组进行SSR引物多态性频率分析。结果表明:在1243对SSR引物中共筛选到多态性引物383对,多态性频率为31.10℅;127对条带差异不清晰;无遗传多态性共有733对,占总筛选引物数的59.35%。其中BARC,GWM,CFA的多态性较高,多态性频率分别为34.98%,34.72%,36.49%;CWEM多态性较差,多态性频率仅为8.89%。多态性引物在不同染色体组间分布频率不同,A为41%,B为35%,D染色体组上多态性较少为24%。Simple sequence repeat （SSR） molecular marker was used to analyze the frequency of polymorphism of wheat varieties- Flinor and Mingxian169. The results followed that： 1243 SSR markers were used to investigate DNA polymorphism of the two cuhivars, 383 markers could express the polymorphic and cover 31.10%, 127 markers had no clear band and 733 markers had no polymorphic and covered 59.35%. The polymorphism of BARC, WMS and CFA was higher with 34.98%, 34.72% and 36.49%, respectively, while the polymorphism of CWEM was lower with 8.89%. The distribution frequency of polymorphic markers was different among the chromosome in the A, B and D genome, the highest in a genome （41%）, lower in B genome （35%） and the lowest in D genome （24%）.

关 键 词：小麦 染色体组 微卫星分子标记 多态性频率 

分 类 号：S512.1[农业科学—作物学]