重组人干扰素γ联合弓形虫STAg鼻内免疫小鼠的免疫保护作用  

Immuneprotective Effect of Intranasal Immunization with Recombinant Human IFNγ and Soluble Tachyzoite antigen of Toxoplasma gondii in Mice

作　　者：孟晓丽[1] 弓鸿飞[1] 殷国荣[1] 刘红丽[1] 王海龙[1] 刘美娜[1] 

机构地区：[1]山西医科大学医学寄生虫学研究所寄生虫学教研室,太原030001

出　　处：《中国生物制品学杂志》2009年第5期483-485,492,共4页Chinese Journal of Biologicals

基　　金：国家自然科学基金(30640057);山西省青年科技基金(20051045)

摘　　要：目的观察重组人干扰素γ(IFNγ)联合可溶性速殖子抗原(STAg)鼻内免疫小鼠诱导的黏膜和系统免疫应答及抗弓形虫感染作用。方法将BALB/c小鼠随机分为STAg组、STAg+IFNγ组和对照组,分别用20μgSTAg、20μgSTAg+1000UIFNγ和20μlPBS鼻内免疫2次,间隔14d。末次免疫后第10天,用RH株弓形虫速殖子灌胃攻击(4×104个/只),观察小鼠存活情况,并计算存活率,攻击后第43天处死全部小鼠,计数肝、脑组织内弓形虫速殖子虫荷,分离并计数肠上皮淋巴细胞(iIELs)、派伊尔氏结(PP)和脾淋巴细胞,ELISA法检测小鼠粪便弓形虫特异性sIgA和血清IgG水平。结果IFNγ联合STAg鼻内免疫能保护小鼠抵抗弓形虫攻击,小鼠存活率(93%)明显高于STAg组(60%)和对照组(47%);小鼠iIELs(1.81×105)、PP(3.21×107)和脾(3.01×107)淋巴细胞均显著增生,明显高于STAg组和对照组;粪便中sIgA水平(A492=0.435)显著高于对照组(A492=0.047),血清IgG水平(A492=0.233)显著高于STAg组(A492=0.193)和对照组(A492=0.115);肝、脑组织内弓形虫速殖子虫荷显著减少,分别比对照组减少80.90%和64.50%。结论IFNγ联合STAg鼻内免疫效果优于单独STAg免疫,IFNγ能协同STAg有效诱导黏膜和系统免疫应答,提高弓形虫攻击小鼠的存活率,降低肝、脑组织内虫荷。Objective To observe the mucosal and systemic immune responses as well as anti-infectious immunity against Toxoplasma gondii induced by intranasal immunization with recombinant human IFNy and soluble tachyzoite antigen （STAg） of Toxoplasma gondii in mice. Methods BALB/c mice were divided into STAg, STAg ＋ IFNy/ and control groups and immunized i.n. with 20 μg of STAg, 20 μg of STAg ＋ 1 000 U IFNy and 20 μl of PBS for 2 times at an interval of 14 d respectively. The mice were challenged intragastrically with tachyzoite of Toxoplasma gondii strain RH on day 10 after the 2nd immunization, 4 x 104 tachyzoites per mouse, and observed for survival, based on which the survival rate was calculated. All the mice were killed on day 43 after challenge and counted for tachyzoites in liver and brain tissues, and their intestinal intraepithelial lymphocytes （iIELs） and lymphocytes in Peyer patches （PP） and spleen were isolated and counted. The Toxoplasma gondii-specific sIgA level in feces and IgG level in sera were determined by ELISA. Results The intranasal immunization with IFNy and STAg protected the mice against the challenge with tachyzoites of Toxoplasma gondii. The survival rate of mice in STAg ＋ IFNy group （93%） was significantly higher than those in STAg （60%） and control （47%） groups. The counts of ilELs （1.81 × 105） as well as lymphocytes in PP （3.21 × 107） and spleen （3.01 × 107） of mice in STAg ＋ IFNy group were also significantly higher than those in STAg and control groups. The sIgA level （A492） in feces of mice in STAg ＋ IFNy group （0. 435） was significantly higher than that in control group （0. 047）, while the IgG level （A492） in sera （0. 233） was significantly higher than those in STAg （0. 193） and control （0. 115） groups. Compared with those in control group, the numbers of tacbyzoites in liver and brain tissues of mice in STAg ＋ IFNy group decreased by 80. 90% and 64. 50% respectively. Conclusion The immune effect of intrana

关 键 词：弓形虫 可溶性速殖子抗原 干扰素y 鼻内免疫 黏膜免疫 黏膜佐剂 

分 类 号：R382.5[医药卫生—医学寄生虫学] R392[医药卫生—基础医学]