蓖麻毒素和相思子毒素蛋白质芯片检测方法的建立  

Development of Protein Chip Technique for Detection of Abrin and Recin

作　　者：路浩[1] 王兴龙[2] 李晓艳[2] 郎需龙[2] 唐婕[2] 张付贤[1] 王英超[1] 党源[1] 

机构地区：[1]吉林大学畜牧兽医学院,长春130062 [2]军事医学科学院军事兽医研究所,长春130062

出　　处：《中国生物制品学杂志》2009年第5期508-510,共3页Chinese Journal of Biologicals

摘　　要：目的建立蓖麻毒素和相思子毒素蛋白质芯片检测方法。方法制备并纯化蓖麻毒素和相思子毒素单克隆抗体。采用竞争免疫法检测抗原,建立检测蓖麻毒素和相思子毒素的蛋白质芯片检测方法。应用蛋白质芯片检测两种毒素的模拟样品。结果应用蛋白质芯片检测蓖麻毒素、相思子毒素和两种毒素混合的模拟样品时,在其相应区域均没有荧光信号,结果为阳性,与实验设计相符。结论已成功建立了蓖麻毒素和相思子毒素蛋白质芯片检测方法,该方法具有广阔的应用前景。Objective To develop a protein chip technique for detection of abrin and recin. Methods The McAbs against abrin and recin were prepared and purified, based on which a protein chip for detection of antigens by competitive immunoassay was prepared and used for detection of mock samples of abrin and recin. Results No fluorescent signals were observed in corresponding regions of mock samples of abrin, recin and their mixture by using the prepared protein chip, indicating positive results consistent with those designed. Conclusion The protein chip technique for detection of abrin and recin was successfully developed, which was of a broad Drospect in application.

关 键 词：蓖麻毒素 相思子毒素 蛋白质芯片 

分 类 号：S-3[农业科学]