长春花叶片内源激素及黄酮与花芽分化的关系  

Correlations of Endogenous Hormones and Flavonoids and Flower Bud Differentiation in Leaves in Catharanthus roseus

作　　者：马书荣[1] 祖元刚[1] 

机构地区：[1]东北林业大学,哈尔滨150040

出　　处：《东北林业大学学报》2009年第5期72-73,共2页Journal of Northeast Forestry University

基　　金：教育部重点项目基金(104191)资助

摘　　要：为了探讨内源激素及黄酮与长春花花芽分化的关系,采用酶联免疫吸附法(ELISA),研究了长春花幼苗由营养生长向生殖生长转变过程中叶片内源激素及黄酮质量分数的动态变化。结果表明:长春花幼苗叶片IAA、GA3、ZR的质量分数均在第一个花芽分化时降低,而ABA质量分数升高,表明内源激素均与第一个花芽分化有密切关系。叶片ABA/ZR、GA3/ZR、IAA/ZR、ABB/IAA和ABA/GA3也是在第一个花芽分化时达到峰值,之后迅速降低,说明向生殖生长转变时叶片内源激素间的平衡关系。叶片黄酮质量分数在第一个花芽分化时期明显下降,部分黄酮通过枝输出消耗于第一个花芽分化。幼苗向生殖生长转变过程中,黄酮起着一定的调控作用。The dynamic changes of endogenous hormones and flavonoids in Catharanthus roseus were studied by ELISA method as plants change from vegetative to reproductive stages, aiming to discuss the relationships between endogenous hormones and flavonoids and flower bud differentiation. During the process of the first flower bud differentiation, the contents of indolylacetic acid（IAA）, gibberellin（ GA3 ） and zeatin（ZR） in the leaves of C. roseus decreased evidently but abscisic acid （ABA） increased, which indicate that the first flower bud differentiation is closely related to ABA, GA3, ZR and IAA. Moreover, ABA/ZR, GA3/ZR, IAA/ZR, ABB/IAA and ABA/GA3 in leaves obviously increased at the first flower bud differentiation stage, and peaked when flower bud differentiation was basically completed, and then decreased significantly, which shows the balanc.e among different endogenous hormones. The contents of flavonoids in the leaves decreased evidently at the first flower bud differentiation stage, implying that part flavonoids in the leaves were transplanted and consumed at the first flower bud differentiation stage. Flavonoids play an important role in regulating and controlling the change process from vegetative growth to reproductive growth for C. roseus.

关 键 词：长春花 花芽分化 内源激素 黄酮 

分 类 号：Q946.885[生物学—植物学]